Robert Page's side have been waiting for months to know which team stands in their way of creating history following a 2-1 win over Austria in March.
But it was Ukraine who confirmed their spot in the playoff final by beating Scotland 3-1 at Hampden Park after the initial fixture was postponed due to the countries invasion by Russia.
Wales only have one World Cup appearance to their name which came all the way back in 1958 when a 17-year-old Pele scored as Brazil dumped them out in the quarters.
Ukraine meanwhile also have only qualified once since breaking away from the Soviet Union as they similarly reached the quarters in 2006.
Wales v Ukraine: Date and start time
This World Cup playoff final game will take place on Sunday, June 5.
Kick-off at the Cardiff City Stadium is scheduled for 5pm.
The winner of this clash will be placed into Group B alongside England, Iran and USA.
Wales v Ukraine: TV channel and live stream
This match will be broadcast live on Sky Sports Main Event and Sky Sports Football with live coverage starting from 4pm.
Sky Sports customers can live stream this via the app using their mobile, tablet or computer devices.
If not, you can purchase a Sky Sports Day Pass from NowTV for £9.99.
To tune in to talkSPORT or talkSPORT 2 through the website, click HERE for the live stream. You can also listen via the talkSPORT app, on DAB digital radio, through your smart speaker and on 1089 or 1053 AM.
Wales v Ukraine: Team news
Wales Squad
Goalkeepers: Hennessey, Ward, A Davies
Defenders: Davies, Rodon, Mepham, Gunter, Norrington-Davies, Roberts, N Williams, Denham
Midfielders:Allen, Morrell, Ampadu, Smith, Ramsey, Levitt, Matondo, Thomas, Colwill, Wilson, J Williams, Burn
Forwards: Bale, James, Harris, Moore, Johnson
Wales v Ukraine: What has been said?
Former Wales striker Rob Earnshaw claims the Dragons must "put aside their compassion" for Sunday's opponents when the game kicks off.
"As a Wales fan and wanting Wales to go through you've obviously got mixed emotions, because it's the compassion you feel for Ukraine," Earnshaw told Radio Wales Breakfast.
"But what I feel and I think what most people in Wales will feel on Sunday is we're going to put that aside, because this is a football match and we're in competition in football for those 90 minutes.
"It is very tough when you're faced with something like this because it's about humanity in this moment and I think everyone has felt so much for Ukraine. They feel the pain, they feel the pain of a country at war."
Former Ukraine striker and manager Andriy Shevchenko added: "We need to play for the fans, for the whole of Ukraine, for those at home, for those defending the country and for those who left the country."