Spurs came from behind to beat Rangers last time out thanks to a brace from Harry Kane as they continue to step up their preparations.
The England captain will now return to action against his former manager Mourinho, who he scored 45 goals in 62 games under.
Tottenham remain the only club of the Special One's career where he failed to deliver silverware after he was sacked days before the 2021 Carabao Cup final defeat to Manchester City.
Tottenham vs Roma: Date and start time
This pre-season friendly will take place on Saturday, July 30.
Kick-off at the 30,780-capacity Sammy Ofer Stadium in Haifa, Israel will take place at 7:15pm UK time.
Tottenham vs Roma: TV channel and live stream
SPURSPLAY will have the game available to buy through their membership, which costs £45 for the year.
talkSPORT will also have updates from the clash in Israel while talkSPORT.com will be on hand for all the post-match reaction.
To tune in to talkSPORT or talkSPORT 2 through the website, click HERE for the live stream. You can also listen via the talkSPORT app, on DAB digital radio, through your smart speaker and on 1089 or 1053 AM.
Tottenham vs Roma: Team news
Spurs have a decision to make over Ben Davies after he suffered an ankle knock against Sevilla and missed the win over Rangers.
However, Conte handed all four of Yves Bissouma, Ivan Perisic, Clement Lenglet and Djed Spence debuts in that game and some may be in line to start here.
Matt Doherty was rested at Ibrox and should be back involved while Kane will face off against England team-mate Tammy Abraham.
As for Roma, Paulo Dybala as an unused substitute in the 1-1 draw with Nice on Saturday and could be in line to feature against Spurs - who he was linked with joining before staying in Italy.
Tottenham vs Roma: What has been said?
Tony Cascarino insists Tottenham will not be 'Spursy' anymore thanks to a total transformation under Antonio Conte.
"They are not going to be Spursy anymore," said the Weekend Sports Breakfast host on talkSPORT.
"When Antonio Conte first got the job, it was quite clear he didn't agree with the direction of the football club.
"It got to early February and they lost to Wolves and Southampton in the Premier League.
"He was clearly absolutely furious. Then they lost to Burnley and he had a total meltdown.
"That was the moment their whole season changed. He went after everything at the club.
"He's a manager who doesn't need a job: he does it because he's addicted to it, he loves it and he demands the highest level.
"It must be very refreshing for Spurs fans."