Nuno Espirito Santo's side survived a scare against Pacos de Ferreira to book their spot in the group stages, prevailing 3-0 at home after a 1-0 first-leg defeat in the playoff round.
However, Spurs enter Thursday's game on the back of a humbling Premier League result, with Crystal Palace easing to a 3-0 win over the 10-man Lilywhites on Saturday lunchtime.
Here, Sports Mole rounds up all of Tottenham's latest injury and suspension news ahead of the contest.
Son Heung-min
Status: Major doubt
Type of injury: Calf
Possible return date: September 16 (vs. Rennes)
Son Heung-min returned to Tottenham following the international break nursing a calf issue and was not involved against Palace. Santo remained hopeful over his availability for this game in his post-match press conference, but he will most likely be spared for the visit of Chelsea next weekend.
Eric Dier
Status: Major doubt
Type of injury: Knee
Possible return date: Unknown
Eric Dier was only on the field for 12 minutes against Palace before having to be withdrawn with a suspected knee problem. Nuno was unable to deliver an update on the defender after the game, but it would be a surprise to see him feature here.
Davinson Sanchez
Status: Out
Type of injury: Other
Possible return date: September 19 (vs. Chelsea)
Davinson Sanchez is one of three South American Tottenham players currently at a training camp in Croatia following the international break, and the Colombian defender will not be available here.
Giovani Lo Celso
Status: Out
Type of injury: Other
Possible return date: September 19 (vs. Chelsea)
As is the case with Sanchez, Giovani Lo Celso is also out of the country after landing himself in hot water by joining up with Argentina and will miss this game.
Cristian Romero
Status: Out
Type of injury: Other
Possible return date: September 19 (vs. Chelsea)
Cristian Romero completes the trio of South American players keeping fit in Croatia, and the defender will return to England alongside his teammates next weekend.
Ryan Sessegnon
Status: Major doubt
Type of injury: Knock
Possible return date: September 16 (vs. Rennes)
Ryan Sessegnon pulled out of the Under-21 England squad with a knock and did not make the trip to Selhurst Park, so the left-sided player is a major doubt for a contest in which he would have hoped to earn some valuable minutes.
Steven Bergwijn
Status: Major doubt
Type of injury: Ankle
Possible return date: September 16 (vs. Rennes)
Steven Bergwijn returned from international duty with the Netherlands nursing an ankle problem, and Santo is 'hopeful' that the attacker will be fit for this week, although his participation is far from certain.
SPURS' SUSPENSION LIST
Japhet Tanganga saw red against Crystal Palace but is available here, and Spurs have no further suspension concerns for the trip to Rennes.