The Citizens have already qualified for the last 16 as Group G winners, while Sevilla are guaranteed to finish in third spot and drop down into the Europa League.
With Erling Braut Haaland not involved as he continues to recover from ligament damage, Julian Alvarez is handed another start in attack, while captain Ilkay Gundogan, Aymeric Laporte and Jack Grealish have also retained their places in the first XI following last weekend's 1-0 Premier League win at Leicester City.
Seventeen-year-old right-back Rico Lewis has been handed his first senior start for the Citizens, joining Laporte, Ruben Dias and Sergio Gomez in the back four, while Stefan Ortega is handed another start in goal ahead of Ederson after making his debut for the club against Borussia Dortmund last week.
Two more academy graduates in Cole Palmer and Phil Foden will be providing the creativity in midfield alongside Gundogan, with star playmakers Kevin De Bruyne and Bernardo Silva both on the substitutes' bench.
Alvarez will be joined in attack by Grealish and Riyad Mahrez - the latter of whom has missed two penalties in his last two Champions League matches, so the Algerian is unlikely to step up from 12 yards if City win another spot kick this evening.
As for Sevilla, former Man City defender Karim Rekik is handed a start in the back four, while another former Citizens man in Jesus Navas is on the substitutes' bench.
Manchester City starting lineup: Ortega; Lewis, Dias, Laporte, Gomez; Palmer, Gundogan, Foden; Mahrez, Alvarez, Grealish
Subs: Ederson, Carson, Stones, Ake, Cancelo, Rodri, De Bruyne, Bernardo, Wilson-Esbrand
Sevilla starting lineup: Bono; Montiel, Marcao, Rekik, Acuna; Jose Angel, Delaney, Rakitic; Isco, Mir, Gomez
Subs: Dmitrovic, Alberto, Telles, Dolberg, Gudelj, Suso, Jordan, Januzaj, Navas, Lamela, C. Alvarez, Salas