The visitors, meanwhile, have won one and lost one game in Europe so far, after falling to their former star striker Erling Haaland's late winner at the Etihad Stadium last time out.
Match preview
For Sevilla, who have finished fourth three seasons running in La Liga under former Spain boss Julen Lopetegui, it has been an incredibly disappointing start to the season.
Saturday's 2-0 defeat at home to Atletico Madrid left them in 17th position in La Liga, having only won one of their seven league games so far this campaign.
Despite dominating possession with 61% of the ball, Lopetegui's side never looked particularly likely to break Atletico's defensive wall down, with goals from Marcos Llorente and Alvaro Morata either side of half-time handing their team an important victory.
Having failed to beat Copenhagen away from home in a dour 0-0 draw before the international break, there is significant pressure on Sevilla to beat Dortmund on Wednesday, otherwise they are going to have an uphill task to progress from Group G.
However, based on current form, dropping down to the Europa League may not be the worst thing in the world for them this season, especially having won Europe's secondary competition in this manner as one of their record six-time triumphs in the competition in 2015-16.
Dortmund, meanwhile, suffered a 3-2 defeat to FC Koln in the Bundesliga at the weekend, falling behind Bayern Munich in the table ahead of their clash at the Westfalenstadion this weekend.
However, Edin Terzic's side's attention must quickly return to the Champions League, where they will realistically be hoping to snatch second spot in Group G behind current leaders Manchester City.
BVB were incredibly unfortunate to lose to the Citizens in their last European match, with Jude Bellingham's 56th-minute header providing them with a surprise lead to hold onto.
They looked comfortable in doing so, too, with Man City failing to have a single shot on target until John Stones's crashing effort from distance brought his side level in the match, with deputy goalkeeper Alexander Meyer seemingly taken by surprise by the England defender electing to shoot.
The script was written for Haaland to make his mark against his former club and - as ever - the Norwegian striker did not fail to deliver, as he acrobatically met Joao Cancelo's superb cross to volley Man City into the lead and claim a precious three points for his new team.
Having comfortably swatted Copenhagen aside on matchday one, Dortmund will still be on course to qualify from Group G as long as they can avoid defeat in Seville in midweek.
Sevilla Champions League form:
L
D
Sevilla form (all competitions):
L
L
W
D
D
L
Borussia Dortmund Champions League form:
W
L
Borussia Dortmund form (all competitions):
W
W
L
L
W
L
Team News
Sevilla will travel without Oliver Torres, who was not included in their Champions League squad, while Tecatito, Karim Rekik and Jesus Corona are expected to miss out through injury.
Alex Telles, who is on loan from Manchester United, should come into the side at left-back, while Joan Jordan, Ivan Rakitic and Papu Gomez all provide different options to bolster the midfield area.
Dortmund, meanwhile, have a whole host of absentees, with Marius Wolf, Mats Hummels, Giovanni Reyna, Marco Reus, Gregor Kobel, Jamie Bynoe-Gittens, Mateu Morey, Mahmoud Dahoud, Nico Schulz and Sebastien Haller all unlikely to make the trip to Spain.
Ahead of Der Klassiker on Saturday, Terzic is unlikely to take any risks with doubtful players, while Emre Can, Thorgan Hazard and Youssoufa Moukoko could all come into the starting XI to freshen things up from the weekend.
Sevilla possible starting lineup:
Bono; Carmona, Nianzou, Marcao, Telles; Jordan, Delaney, Rakitic; Gomez, En-Nesyri, Isco
Borussia Dortmund possible starting lineup:
Meyer; Meunier, Sule, Schlotterbeck, Guerreiro; Can, Bellingham; Moukoko, Brandt, Hazard; Modeste
We say: Sevilla 1-1 Borussia Dortmund
Given the amount of players Dortmund are likely to have unavailable for this fixture - and the importance of Der Klassiker at the weekend - we can envisage the visitors being extremely content to leave Seville with a point on Wednesday.
Sevilla have started the season in terrible form and may not be massively disappointed with avoiding defeat either, so we can envisage a share of the spoils in midweek.