The two sides played out an eventful 1-1 draw in Rotterdam on Thursday, meaning that we are no closer to knowing which club will qualify for next season's Europa Conference League.
Match preview
FC Twente appeared to be heading for a 1-0 defeat in the first leg, but a 93rd-minute equaliser from Ramiz Zerrouki saw the visitors snatch a share of the spoils at Sparta Stadium.
In fairness, the Tukkers dominated the first leg, enjoying the lion's share of the ball while racking up 20 shots, although Ron Jans's men failed to make the most of their dominance, as they managed only three attempts on target.
Thanks to Thursday's draw, Twente head into the second leg on an eight-game unbeaten streak, having ended the regular season with a run of four straight wins before sealing back-to-back victories over Heerenveen to reach the European playoff final.
When you look at the bigger picture, the Pride of the East have lost just one of their previous 14 encounters, picking up nine victories while scoring at least three goals on nine occasions.
It is also worth noting that Twente have been immense at De Grolsch Veste this season, suffering a solitary defeat in 22 home games across all competitions, so they will be confident of completing the job this weekend.
When Vito van Crooij scored a second-half penalty to give Sparta Rotterdam the lead in the first leg, it looked as though the hosts would snatch an underserved victory, but their backline eventually cracked late on.
Still, after finishing the contest with 36% possession and two shots on target to their name, De Kasteelheren can count themselves fortunate to have scraped a draw at Sparta Stadium.
It would not be unfair to say that Sparta Rotterdam have stuttered somewhat in recent weeks, as they required a penalty shootout to overcome FC Utrecht in the European playoff semi-finals.
As a result, Maurice Steijn's men enter the second leg on a run of three wins, two draws and three losses, so they will be eager to rediscover the consistency that saw them register their highest Eredivisie finish since 1984-85.
Sparta Rotterdam can take confidence from their recent away record, however, as they have lost just one of their previous seven away games, notching five wins while scoring at least two goals on five occasions.
FC Twente Eredivisie form:
W
W
W
W
W
D
Sparta Rotterdam Eredivisie form:
L
W
W
W
D
D
Team News
FC Twente look set to be without young centre-back Max Bruns for the second leg, as the 20-year-old has missed the past nine matches due to injury.
Having racked up an impressive 15 goals and 13 assists this season, Czech winger Vaclav Cerny will offer attacking support to lone striker Manfred Ugalde, with Virgil Misidjan operating on the opposite flank.
Ron Jans will likely stick with his favoured 4-2-3-1 formation, meaning that Joshua Brenet, Julio Pleguezuelo, Robin Propper and Gijs Smal will occupy the backline.
As for Sparta Rotterdam, there were question marks over the fitness of Tobias Lauritsen and Mike Eerdhuijzen ahead of the first leg, but both players started on Thursday.
Lauritsen has chalked up 15 goals and six assists this term, while Vito van Crooij has notched 14 goals and 12 assists, so they will continue in attack alongside Japanese forward Koki Saito.
Maurice Steijn is expected to deploy a 4-3-3 formation, with Joshua Kitolano, Arno Verschueren and Younes Namli lining up in midfield.
FC Twente possible starting lineup:
Unnerstall; Brenet, Pleguezuelo, Propper, Smal; Zerrouki, Sadilek; Cerny, Vlap, Misidjan; Ugalde
Sparta Rotterdam possible starting lineup:
Olij; Sambo, Vriends, Eerdhuijzen, Meijers; Kitolano, Verschueren, Namli; Van Crooij, Lauritsen, Saito
We say: FC Twente 2-1 Sparta Rotterdam
Following Thursday's stalemate, FC Twente and Sparta Rotterdam have now played out three draws this season, although the Tukkers could - and probably should - have defeated De Kasteelheren last time out.