A fan ran onto the field of play midway through the first half while the Lionesses prepared to take a free-kick.
They were quickly tackled by security and led away.
The pitch invader appeared to be wearing a t-shirt that was protesting Russia's war in Ukraine.
On the front, it had an image of Vladimir Putin mocked up as Adolf Hitler.
On the back, there was a message that read 'free Ukraine'.
The game was not disrupted for too long and play soon restarted, but the England free-kick came to nothing.
The score was 0-0 at the time of the incident, but Spain went on to take the lead just five minutes later.
Olga Carmona fired home for Spain, driving the ball beyond Mary Earps.
The Lionesses themselves had chances as Lauren Hemp hit the crossbar in the 16th minute.
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