The French centre-back picked up a suspected knee injury in the closing stages of Liverpool's 2-0 win over Real Madrid on Wednesday night.
Konate required medical treatment late on after being fouled by Los Blancos starlet Endrick.
His teammates crowded around the player, before he got back to his feet.
But the pain was there for all to see following the full-time whistle as he grimaced on his way to the tunnel.
He even had to stop during his walk to hold his right knee in worrying scenes.
READ MORE ON LIVERPOOL
Real Madrid create unwanted history with defeat to Liverpool as Reds equal club record
Gareth Bale suggests theory for Mohamed Salah's public Liverpool contract statement
Konate wasn't the only Liverpool star to pick up an injury, with right-back Conor Bradley going off with a hamstring knock.
Commenting on the pair, boss Slot insisted it is too soon to assure fans that they will be fit to face Premier League title rivals City.
He said of the duo: "It's difficult to judge so soon after the game.
"I'm really hoping that both will be available to play."
Most read in Champions League
Bradley bullies Mbappe in statement Liverpool showing to put Alexander-Arnold on notice
EA FC 25 RTTK upgrade tracker: How it works and dates explained for Road to the Knockouts
Carragher in stitches at contract negotiation dig amid Salah 'selfish' furore
Real Madrid create unwanted history with defeat to Liverpool as Reds equal club record
Konate has started in the last 11 top-flight games having starred off the bench when replacing Jarell Quansah at half-time on the opening day of the season away at Ipswich.
He has been a huge reason as to why Liverpool sit eight points clear at the top of the table having conceded just eight goals in the division.
And the thought of Konate being unavailable against Pep Guardiola's men proved to be difficult to take for Liverpool fans.
Taking to X, one user posted: "If Konate injury is serious these 3 points [vs Madrid] simply not worth it."
Another messaged: "A Konate injury would completely ruin this night. It's the last thing we need."
A third added: "That Konate injury is not allowing me to be happy with this win."
And this fan simply stated: "That Konate injury could be an absolute disaster for our season…"
Should Konate miss out against City, Quansah would likely come back into the starting XI for his first start since August.