Meanwhile, Croatia are hoping to claim their first win after picking up a point in their tournament opener against Austria.
Match preview
Denmark began their seventh European Under-17 Championship appearance with a 2-0 victory in Monday's clash with Wales.
Chido Obi opened the scoring in first-half stoppage time, before Sofus Johannesen doubled Denmark's advantage in the opening stages of the second period.
They ultimately saw out the remainder of the contest to clinch a third consecutive victory in all competitions and extend their unbeaten run to seven matches.
Having got off the mark with a win, Denmark are looking to advance to the European Under-17 Championship knockout rounds for the fourth time.
They know that a win on Thursday would seal their spot in the quarter-finals even before they face Austria on the final matchday.
Croatia head into Thursday's contest with a point on the board after beginning their campaign with a goalless draw against Austria.
As a result of that scoreline, Croatia have now seen their last five competitive matches produce one win, two draws and two defeats.
Although they have found wins hard to come by, Croatia will be pleased with the fact they have claimed clean sheets in their last two outings.
Croatia will hope a solid backline can help them avoid another group-stage elimination, having failed to reach the knockout rounds in their last two appearances at the European Under-17 Championship.
As a result, they are looking to reach the quarter-finals for the first time since they reached the last eight in the 2015 tournament.
Denmark Under-17s European Under-17s Championship form:
Croatia Under-17s European Under-17s Championship form:
Team News
Denmark boss Jesper Mikkelsen could be tempted to select an unchanged lineup after seeing his side get off to a winning start against Wales.
Arsenal's Obi will be looking to continue his impressive form, having scored 10 goals in 14 appearances for Denmark Under-17s.
As for Croatia, Bruno Durdov, Dukan Ahmeti and Tomas Bakovic are all in contention to start after missing the tournament opener through suspension.
Mislav Cutuk also missed the draw with Wales due to a one-match ban for yellow card accumulation in qualifying, but the forward is expected to be given the chance to lead the line on matchday two.
Denmark Under-17s possible starting lineup:
Breum-Harild; Lutzhoft, Sondergaard, Markmann, Andersen; Moalem, Lassen, Hojer; Johannesen, Hyseni, Obi
Croatia Under-17s possible starting lineup:
Rajic; Mikic, Zebic, Puljic, Mandic; Pajsar, Vojvodic; Durdov, Bakovic, Ahmeti; Cutuk