Meanwhile, the tournament hosts will be hoping to produce a positive response after suffering a heavy defeat in their tournament opener.
Match preview
Cyprus are competing in their first European Under-17 Championship after securing direct entry as the tournament hosts.
As the only debutants in the competition, they were given a harsh reality check in Monday's opening game against the Czech Republic.
Chrysis Michail saw his side ship three goals in a 14-minute period to leave them with a mountain to climb at the break.
Cyprus went on to concede two more goals in the second half as the Czech Republic ran out 5-0 winners at the AEK Arena in Larnaca.
As a result of that heavy defeat, Cyprus will be eliminated with a game to spare if they lose and Serbia take a point from their meeting with Ukraine.
In contrast to their opponents, Serbia will be in high spirits after they get their campaign up and running with a narrow victory over Ukraine.
Dusan Makevic's second-minute effort proved enough to secure Serbia a precious 1-0 victory, leaving them well-placed ahead of the second round of matches.
They are looking to reach the knockout rounds for the third consecutive European Under-17 Championship after reaching the semi-finals in 2022 and the quarter-finals in 2023.
Serbia will secure their place in the last eight if they beat Cyprus and the Czech Republic pick up at least a point in the group's other game.
Jovan Damjanovic's side will be confident they can keep the tournament hosts at bay, having kept clean sheets in three of their last four competitive matches.
Cyprus Under-17s European Under-17s Championship form:
Serbia Under-17s European Under-17s Championship form:
Team News
Cyprus defender Georgios Skafi is in contention to start after featuring as a half-time substitute against the Czech Republic.
Michail could be tempted to make a couple more changes, with Marios Chatzilefteri and Anninos Nikolaou among those who could be considered for a starting place.
As for Serbia, Andrija Maksimovic and Djordje Rankovic will be available for selection after serving one-match bans.
Mihajlo Cvetkovic will continue to spearhead Serbia's attacking efforts, having scored six goals in 14 appearances at Under-17 level.
Cyprus Under-17s possible starting lineup:
Michos; Petoussis, Skafi, R Dimosthenou, K Ioannou, Michalas; Chatzilefteri, Christodoulou; Evangelou, Nikolaou, P Iannou
Serbia Under-17s possible starting lineup:
Jovanovic; Bubanja, Roganovic, Milosavljevic, Stojanovic; Makevic, Maksimovic, Vasilic; Kostov, Rankovic, Cvetkovic