The world no.43 ranked side progressed to the last 16 with a 0-0 draw, holding off Brazil to finish second in Group F behind France.
Brazil took off all-time great Marta with nine minutes to play, and the World Cup great was seen looking utterly stunned, while many of her teammates were in tears.
Jamaica's troops, meanwhile burst into tears of joy, while their coached rolled onto the pitch in shock.
The Caribbean nation needed a crowdfunding campaign to compete at the Women's World Cup and now they have dumped out a giant.
Relying on handouts due to ongoing disputes over payments with the Jamaican Football Federation, the mother of forward Havana Solaun set up a crowdfunding campaign to cover the costs of travelling to, and staying at, the New Zealand and Australia tournament.
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Their target of $100,000 [£78,000] was not met, but a serge of donations equating to £15,000 will likely be added to as the heart-warming story progresses.
Jamaica manager Lorne Donaldson was in shock post-match, calling it "one of the best feelings of my life" after he roly-polied onto the pitch at full time.
But for every tear of joy, there was a tear of sadness from Brazil, who have never won the tournament despite often entering as one of the favourites.
Much of their international success has been down to the legendary Marta, who was featuring in her sixth World Cup at the age of 37.
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She had already announced the 2023 tournament would be her last, but could have hardly expected it to end so early at the group stage, looking utterly stunned on the bench.
Then, while being consoled by an opponent, the tears finally came while Jamaica enjoyed their moment in the sun.