Villarreal claimed a fine 1-0 win in the first-leg of their quarter-final clash to hold a slight advantage in the tie.
Arnaut Danjuma fired home early for Unai Emery's side and the Spaniards will be eager to get the job done this evening.
But that won't be easy against Bayern.
Julian Nagelsmann's men were held to a 1-1 draw by Red Bull Salzburg in their first-leg tie in the last round but the German giants went on to win 8-2 on aggregate.
Bayern are also top of the Bundesliga again and will be confident of turning around their clash with Villarreal.
Bayern Munich v Villarreal: Date and start time
This Champions League tie will take place on Tuesday, April 12.
The game at the Allianz Arena is scheduled to kick off at 8pm UK time.
These two last met in the groups stages of the 2011/12 Champions League campaign with Bayern winning both matches with an aggregate scoreline of 5-1.
Bayern Munich v Villarreal: TV channel and live stream
This match will be broadcast on BT Sport 3 with coverage starting at 7:15pm
BT Sport customers can live stream this via the app using their mobile, tablet or computer devices.
Bayern Munich v Villarreal: Team news
Bayern duo Bouna Sarr and and Eric Maxim Choupo-Moting are set to miss out.
Lucas Hernandez, Alphonso Davies and Kingsley Coman were all rested at the weekend and should return for this game.
Villarreal made 10 changes in La Liga over the weekend.
The likes of Giovani Lo Celso, Juan Foyth and Francis Coquelin had their last game off and will be back in the starting line-up.
Bayern Munich v Villarreal: What has been said?
Villarreal head coach Unai Emery: "We're aware that it will be tough, but I'm convinced that we can compete and find the result against the tough demands of an opposition who are the favourites, despite the first-leg result.
"They're one of the favourites to win the Champions League.
"We're preparing for the game. We know that it will be difficult, we want to execute our game well and have chances. This team has grown a lot. We need to adapt to the demands of the opposition, show personality and be ourselves.
"We want to be focused throughout the match. We have to play with the resources we have.
"We're not satisfied with reaching where we have, but we want more and to go through. We know it is tough, but we want to find our moments in a demanding situation."