Vinicius has been out since the end of August with a hamstring issue, while Guler is yet to make his debut following a knee injury, which required a minor operation.
The duo have been in training ahead of the contest, and it will be revealed on Saturday whether the pair will be in the squad, although neither are likely to be considered for a start.
Dani Carvajal's muscular issue is more serious than first thought, so he will again miss out, while Thibaut Courtois and Eder Militao remain long-term absentees due to serious knee injuries.
Lucas Vazquez should again get the nod at right-back, with Joselu set to continue alongside Rodrygo in attack.
The big talking point in terms of team selection comes in midfield, with head coach Carlo Ancelotti boasting a number of options in that particular area.
There has been much discussion surrounding Toni Kroos and Luka Modric early this season, with neither now considered a certain starter, and the pair are both expected to start on the bench here.
Jude Bellingham has been the star of the show this season, scoring six times in his first six appearances, and the England international is again set to feature as the number 10.
Ferland Mendy and Dani Ceballos have both recently recovered from injury problems, meanwhile, and the pair should feature in the squad.
Real Madrid possible starting lineup: Kepa; Vazquez, Rudiger, Alaba, F Garcia; Valverde, Tchouameni, Camavinga; Bellingham; Rodrygo, Joselu