The Scottish champions will have the daunting task of having to stop Erling Haaland and Jude Bellingham in their tracks, with the German club surely one of the favourites to win the competition after their slip from the Champions League.
But for Gio Reyna, another one of Dortmund's teenage sensations, the match will be significant for a very special reason.
Rangers are not only part of his DNA, they also shaped his identity.
Reyna's father, former footballer Claudio, gave his son the name Giovanni as a nod to his close friend and former teammate at Rangers, Giovanni van Bronckhorst.
The pair spent two years together at Ibrox, winning two titles and the Scottish Cup under Dick Advocaat.
The football gods certainly work in mysterious ways and the legendary Dutch midfielder will now be tasked with stifling his friend's son, the United States international who has been regarded one of the world's top young talents in recent years.
Dortmund dropped to the Europa League after finishing third in their group.
Ajax and Sporting Lisbon finished in first and second respectively which saw the Bundesliga club drop down to Europe's secondary competition.
Stopping Haaland will be at the forefront of Van Bronchorst's plans.
The Norway international has been one of the world's best players since joining Dortmund in 2020, hitting a staggering 74 goals in 73 games.