Barca Women take on Real Madrid in the second leg of their Champions League quarter-final on Wednesday night.
Tickets for the clash at the 99,354 seater stadium have completely sold out for the occasion, which will make it the highest attendance in the women's game ever.
The match will surpass the attendance at the Wanda Metropolitano in 2019 when Barca defeated Atletico Madrid 2-0, which saw over 60,739 people attend.
It is also set to break the record held at 1999 World Cup at the Rose Bowl in Pasadena, when the USA defeated China in front of 90,195 spectators.
Barca hold a 3-1 lead from the first leg last week in the Spanish capital. Olga Carmona gave Los Blancos the lead, but Barca captain Alexia Putellas equalised with a penalty, before Claudia Pina put them 2-1 ahead. Putellas then added a third in stoppage time to hand her side the healthy advantage.
Barcelona Women have been in scintillating form, having become the first Spanish side to win the Champions League with a 4-0 victory over Chelsea, and also won the league and Copa de la Reina in the 2020-21 season.
This campaign, they are eyeing the quadruple and and won the Super Cup with a 7-0 defeat of Atletico. At the start of March, they were also crowned La Liga champions, with seven games to spare.
In all competitions thus far, they have played 36 and won all of them. They've netted a staggering 138 goals in the process and conceded just seven.
The women played at the Camp Nou last season, but COVID-19 meant the game went ahead behind closed doors.
Barca's men's team have recorded a highest attendance of over 86,400 people this season, and the average crowd has been around 50,000.
Fans will get the opportunity to see Ballon d'Or winner Putellas in the flesh for the first time at the Camp Nou, who has netted 24 goals in all competitions this season. She is known as 'La Reina', which translates to the 'The Queen' in English.
Real Madrid are yet defeat Barcelona in any competition, losing all eight matches played against them, and have conceded 35 goals while scoring only four.
Putellas is looking forward to the opportunity of playing at the iconic stadium, and believes the time is right for her side to grace the Camp Nou turf.
"I believe the time has come, it's the right time to do so,' Putellas said.
"I didn't picture myself playing in the Nou Camp as a girl as I only saw men playing there. The game can open so many doors for so many girls and I feel proud Barca makes it possible."
"Many girls will dream of being able to play on such a special stage,' Putellas said.
"It could be a before-and-after moment in women's football."