The sides come into the game level, having drawn 1-1 in the first leg in Brazil last Wednesday.
Match preview
Racing Club cruised through the Copa Libertadores group stage with relative ease, winning four of their six games and remaining unbeaten to top the group with 14 points.
That included a 1-0 win over Sao Paulo, with Joaquin Novillo scoring the only goal of the game to see them defeat their round-of-16 opponents.
On the domestic scene, Juan Antonio Pizzi's side find themselves in a mixed run of form, having ended the first phase of the Argentine Premier Division with consecutive wins to finish fourth before being held to a goalless draw in the first game of the second phase against Velez Sarsfield.
With home advantage coming up, La Academia will certainly take confidence from the first leg, with saw them navigate through a tricky away tie and remain level.
In the first leg, Vitor Bueno gave Sao Paulo the lead in the 33rd minute, before Enzo Copetti drew the Argentinian side level on the stroke of half time.
They will now look to record their second victory over their group rivals in the Copa Libertadores this season in order to book their place in the quarter-finals.
That will undoubtedly be a difficult task though, with Sao Paulo desperate to defeat Racing Club after three failed attempts this year.
The first of the two group meeting between the sides saw the Brazilian giants travel to Greater Buenos Aires and come away with a commendable point following a goalless draw.
Meanwhile, their only defeat in the group stage came to the Argentinian side, as two wins over Sporting Cristal and a 2-0 victory against Rentistas saw them finish comfortably in second spot with 11 points from six games.
However, Hernan Crespo's men have had a relatively poor start to the domestic season, currently sitting in 15th spot in the Brazilian top flight with 11 points and just two victories from the opening 12 games.
Those two wins both came in the lead up to the first leg last week, as they defeated Internacional 2-0 and Bahia 1-0 before being beaten 1-0 by Fortaleza last time out.
They will hope to forget their league form and finally get a victory over Racing Club at a crucial time in order to seal their place in the final eight.
Racing Club Copa Libertadores form:
Racing Club form (all competitions):
Sao Paulo Copa Libertadores form:
Sao Paulo form (all competitions):
Team News
Racing Club's star man could again be attacker Tomas Chancalay, who has hit five goals in five Copa Libertadores appearances this season.
Enzo Copetti should continue to lead the line, having hit the equaliser in the away leg.
Nery Dominguez, Mauricio Martinez and Leonardo Sigali will like reform a back three after a solid performance last time out.
Sao Paulo could remain without forward Eder, who was forced off through injury in the first half of the home leg and missed the latest league game as a result.
However, they do have a ready-made attacking replacement in Vitor Bueno, who scored the opening goal almost immediately after coming on in place of Eder.
Racing Club possible starting lineup:
Arias; Dominguez, Martinez, Sigali; Caceres, Mena, Moreno, Miranda, Piatti; Chancalay, Copetti
Sao Paulo possible starting lineup:
Volpi; Costa, Arboleda, Leo; Vinicius, Santos, Liziero, Welington; Bueno; Gomes, Benitez
We say: Racing Club 1-0 Sao Paulo
Having already squared off three times in this year's Copa America, the two sides will certainly know each other's strengths and weaknesses, and with two draws and a narrow Racing Club victory so far, it will definitely be a close encounter.
We see Racing Club just getting over the line on Wednesday to advance to the final eight thanks to their impressive defensive solidity, but the clash could undoubtedly go either way.