While the hosts are guaranteed to finish bottom of Group G, the visitors have already secured their place in the last 16 with a game to spare.
Match preview
Copenhagen are one of only four teams in this season's Champions League who are yet to win in the group stage, after drawing two and losing three of their first five matches in Group G, and they are the only side who have failed to score.
Indeed, both draws have come on home soil against Sevilla and Manchester City, which bodes well for Jacob Neestrup's side ahead of their final group clash with Dortmund. Copenhagen are in fact the only team in European Cup/Champions League history to have played out four successive goalless draws on home soil, including two in the 2016-17 campaign.
In addition, Byens Hold have kept a clean sheet in 60% of their Champions League matches at Parken Stadium, the best percentage of any team in the competition's history to play at least 10 games.
Nevertheless, Copenhagen's Group G fate was sealed last week when they suffered a 3-0 away defeat against Sevilla, a result which means the La Liga side are guaranteed to finish third at their expense and drop down into the Europa League knockout rounds.
Copenhagen responded to their defeat in the South of Spain with a 2-0 away victory over Randers in the Danish Superliga last weekend. The 14-time top-flight champions have endured a difficult start to their title defence so far this season and currently sit 10 points behind league leaders Nordsjaelland after 15 games.
Neestrup's side, who have lost all three previous European meetings with Dortmund, will be seeking to avoid becoming the fourth team in Champions League history to not score a single goal in the group stage after Deportivo La Coruna in 2004-05, Maccabi Haifa in 2009-10 and Dinamo Zagreb in 2016-17.
After failing to progress past the group stage of the Champions League last season, Borussia Dortmund have booked their place in the last 16 with a game to spare this time around courtesy of a goalless home draw with Group G winners Manchester City last week.
BVB are guaranteed to finish as Group G runners-up due to Man City's superior head-to-head record, and their opponents in the knockout rounds will be determined when the draw is made on November 7.
Edin Terzic's side head into Wednesday's dead-rubber fixture with Copenhagen off the back of a 2-1 Bundesliga away victory against Eintracht Frankfurt last weekend. Despite facing 20 shots on goal from Frankfurt, strikes from Julian Brandt and Jude Bellingham helped Dortmund secure maximum points and move to within four points of Union Berlin at the summit.
Indeed, Bellingham has been a standout performer for BVB so far this season, particularly in the Champions League after scoring four goals in five matches. The 19-year-old scored in both away games against Man City and Sevilla, and the midfielder could become just the third teenager after Kylian Mbappe in 2017-18 and Erling Braut Haaland in 2019-20 to score in all three group-stage away fixtures in a single season.
Dortmund secured a 3-0 home win over Copenhagen last month, with Bellingham also on the scoresheet on that occasion, and they have never lost against Danish opponents in major European competition.
Copenhagen Champions League form:
D
L
D
L
D
L
Copenhagen form (all competitions):
D
D
D
D
L
W
Borussia Dortmund Champions League form:
W
L
W
D
D
Borussia Dortmund form (all competitions):
D
L
W
W
D
W
Team News
Copenhagen centre-back Davit Khocholava is suspended after receiving a red card in the dying embers of last week's defeat against Sevilla, so Denis Vavro is set to deputise at the heart of the defence alongside Valdemar Lund.
Akinkunmi Amoo and Zeca are both long-term absentees as they continue to recover from ruptured ligament injuries, while Nicolai Boilesen is ruled out until December with a knee problem.
Versatile attacker Viktor Claesson, who is the club's top scorer so far this season with eight strikes, has played the last three games in centre-midfield and he could continue in the middle of the pitch on Wednesday alongside Lukas Lerager and Marko Stamenic.
As for Borussia Dortmund, Marco Reus (ankle), Thomas Meunier (cheekbone fracture) and Raphael Guerreiro (muscle) all remain out with injuries, while Sebastian Haller is also unavailable but has been spotted training with former club Ajax just three months after undergoing surgery on a testicular tumour.
Mahmoud Dahoud is doubtful with a shoulder problem, so either Salih Ozcan or Emre Can could be handed a start in centre-midfield alongside Bellingham.
The likes of Anthony Modeste, Marius Wolf and Giovanni Reyna, who all began on the bench last weekend, will be pushing to start in attack on Wednesday.
Copenhagen possible starting lineup:
Grabara; Diks, Vavro, Lund, Kristiansen; Lerager, Stamenic, Claesson; Bardghji, Haraldsson, Daramy
Borussia Dortmund possible starting lineup:
Meyer; Sule, Hummels, Schlotterbeck, Hazard; Ozcan, Bellingham; Wolf, Brandt, Reyna; Modeste
We say: Copenhagen 0-2 Borussia Dortmund
Copenhagen will be keen to end their group-stage campaign on a high and at least score their first goal in this season's competition to give the home supporters something to cheer about.