There was huge controversy during the last international break due to the status of some players following their travel to South American.
Spurs lost Argentine pair Giovani Lo Celso and Cristian Romero and Colombian Davinson Sanchez for 10 days once they returned.
The trio, along with Brazil's Emerson Royal, will not miss any games this time around despite heading to red-list countries for World Cup qualifiers.
The Government announced on Friday that players would be allowed to train and play upon their return while isolating at club facilities, providing they were fully jabbed.
Nuno Espirito Santo confirmed that would not happen again following his side's 2-1 win over Aston Villa.
"I can update, they will go and they will return and they will return on different situations," he said.
"They will have to isolate, but we are fortunate enough to have the Lodge and work normally with the team."
Villa, meanwhile, will lose Emi Martinez and Marvelous Nakamba to red-list countries, and Smith said: "I wanted a better solution but it is a real difficult situation involving FA and governments.
"We have to deal with it. The one thing we will have is players back and ready to play against Wolves. We will have them back this time."