The Citizens - who need to win to progress to the playoffs - come into their final league phase battle on the back of a 3-1 Premier League win over Chelsea, where Khusanov made his first start following his January arrival from Lens.
However, the Uzbekistan international was at fault for Noni Madueke's opener just three minutes in and was taken off early in the second half for John Stones, but he was given a rousing reception from the Etihad faithful as he was withdrawn.
With both Ruben Dias (abductor) and Nathan Ake (muscle) working their way back from issues, Guardiola ought to restore Stones to the first XI alongside Manuel Akanji, Josko Gvardiol and Ederson at the back.
Matheus Nunes has been filling in at right-back since Kyle Walker's move to AC Milan, but a fresh-legged Rico Lewis could now be given another opportunity to impress as Guardiola shuffles the pack.
Rotation could extend to midfield and attack too, as Kevin De Bruyne will no doubt have his eyes on Ilkay Gundogan's spot, while Savinho and Jack Grealish are both contenders to replace Omar Marmoush, who was a bright spark on his debut.
The Egypt international is both an option to play alongside and in place of Erling Haaland, but the Scandinavian should reprise his role on Wednesday, where he will look to build on an astonishing record of eight goals from four Champions League games against Belgian clubs.
Manchester City possible starting lineup:
Ederson; Lewis, Stones, Akanji, Gvardiol; Silva, Kovacic; Foden, De Bruyne, Savinho; Haaland
Written by
Ben Knapton