The hosts are sitting in third place in Group A, while the visitors are languishing at the bottom of the standings without a point to their name.
Match preview
Wolfsburg experienced a difficult start to the group phase, with the German side failing to take anything from their first two fixtures.
After losing 1-0 in their away meeting with Roma, Wolfsburg went on to fall to a 2-0 home defeat against record European champions Lyon on matchday two.
However, Tommy Stroot's side have managed to produce a positive response to that Lyon defeat, having put together a five-game winning run in all competitions, including a dominant victory in last week's away clash with Galatasaray.
Rebecka Blomqvist netted a hat-trick alongside goals from Joelle Wedemeyer and Vivien Endemann to clinch a 5-0 victory in Istanbul, moving them to within three points of second-placed Roma.
Wolfsburg will fancy their chances of enjoying another successful outing on Wednesday, especially as they won six of their last seven competitive home matches.
Galatasaray are one of four teams yet to pick up a point in the group stage, having experienced a difficult start to their first-ever appearance in the main draw.
After losing 3-0 to Lyon in their group opener, the Turkish champions fell to a heavy 6-0 defeat in their home meeting with Italian champions Roma.
Galatasaray conceded five more goals in their recent clash with Wolfsburg, leaving them with the worst defensive record in this season's group stage.
With six points separating them from the top two, Galatasaray will be eliminated with two games to play if they lose to Wolfsburg, or if they draw and Roma manage to avoid defeat in their away meeting with Lyon.
Their hopes of claiming a positive result will be tempered by the fact they have lost their last three competitive away games against Lyon, Fenerbahce and Besiktas.
Wolfsburg Women Women's Champions League form:
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Wolfsburg Women form (all competitions):
Galatasaray Women Women's Champions League form:
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Galatasaray Women form (all competitions):
Team News
Wolfsburg are unable to call upon the services of Caitlin Djikstra, Diana Nemeth, Camilla Kuver and Kristin Demann.
Sarai Linder, Janina Minge and Blomqvist could be recalled to the starting lineup after they dropped down to the bench for Sunday's league win over Turbine Potsdam.
Lineth Beerensteyn is likely to feature in the Wolfsburg forward line, with the attacker looking to continue her impressive form after scoring seven goals in her last 10 appearances.
As for the visitors, they are expected to line up with a back four of Berna Yeniceri, Blessing Demehin, Jazmin Jackmon and Ecem Cumert.
Kristina Bakarandze featured as a substitute on matchday three, but the midfielder is in contention to make her third start of the group phase.
Andrea Staskova will offer one of the visitors' main goal threats, having scored seven times in five European appearances this season.
Wolfsburg Women possible starting lineup:
Frohms; Wilms, Hendrich, Wedemeyer, Linder; Huth, Lattwein, Minge; Beerensteyn, Brand, Blomqvist
Galatasaray Women possible starting lineup:
Yaman; Yeniceri, Demehin, Jackmon, Cumert; Karabulut, Ecem, Topcu, Bakarandze; Usme; Staskova