The White and Violets, who finished second in last season's Segunda Division to secure a return to the top flight, opened their 2024-25 La Liga campaign with a 1-0 success over Espanyol.
Raul Moro netted the only goal of the contest last time out, and he is expected to continue in a wide area, with Amath Ndiaye set to feature on the opposite side.
Real Valladolid lined up in a 4-5-1 formation against Espanyol, and that is again expected to be the case here, with Mamadou Sylla set to be given the nod in the final third of the field.
The away side's squad will be boosted by the return of Andre Ferreira following a suspension, while Kenedy is being backed to shake off a fitness problem to potentially feature.
However, Pezzolano will definitely be missing Anuar Tuhami due to a hernia issue.
Real Valladolid have signed two players from Real Madrid this summer, with Juanmi Latasa arriving on a permanent basis and Mario Martin on loan, and there is a chance that both could feature on Sunday.
Real Valladolid possible starting lineup: Hein; Perez, Sanchez, Boyomo, Rosa; Ndiaye, Perez, Comert, Amallah, Moro; Sylla