Carlo Ancelotti's side will enter the contest off the back of a 1-1 draw against Las Palmas, with Vinicius Junior's second-half strike cancelling out an opener from Alberto Moleiro.
Los Blancos have only won one of their opening three league games of the season, with a total of five points leaving them in fourth position in the table.
Here, Sports Mole rounds up Real Madrid's injury and suspension news ahead of their contest with Real Betis, who occupy 14th spot in the division with two points from two games.
Jude Bellingham
Status: Out
Type of injury: Muscle
Possible return date: September 28 (vs. Atletico Madrid)
Bellingham suffered a muscular injury during a training session ahead of the recent clash with Real Valladolid, and the England international will not be available until the end of next month.
David Alaba
Status: Out
Type of injury: Knee
Possible return date: Unknown
Alaba suffered an ACL injury towards the end of last year, which required an operation, and he then underwent further surgery in the middle of May. The centre-back has started running this week, but he will not be available for selection until the latter stages of this year.
Eduardo Camavinga
Status: Out
Type of injury: Knee
Possible return date: September 21 (vs. Espanyol)
Camavinga suffered a knee injury in a training session ahead of the UEFA Super Cup, and the France international is likely to be out until the clash with Espanyol on September 21.
REAL MADRID'S SUSPENSION LIST
Real Madrid have no players suspended for their clash with Real Betis.