Rosengard are yet to pick up a point in the group stage, while Benfica are three points adrift of Bayern Munich, who sit in second place.
Match preview
Rosengard booked their place in the group stage with a 4-1 aggregate victory over Brann in qualifying, but they the Swedish champions have struggled to make the step up in the main draw.
Despite taking the lead in their group opener against Bayern Munich, Rosengard were edged out via a 2-1 scoreline.
They were then beaten 4-1 by Barcelona on matchday two, before they were narrowly beaten by Benfica in their most recent Champions League game.
Without a point to their name, Rosengard are currently bottom of Group D with six points separating them from the top two.
Rosengard's domestic campaign ended at the beginning of November, and as a result, they have had two weeks to prepare for Wednesday's meeting with Benfica.
Meanwhile, Benfica could move level on points with Bayern if they beat Rosengard and the German champions lose to Barcelona.
The Portuguese champions began the Champions League group stage with a demoralising 9-0 defeat to Barcelona, before they lost 3-2 to Bayern Munich despite taking a two-goal lead.
After spurning the chance to claim all three points against Bayern, the Portuguese champions managed to record their first victory of the group stage against Rosengard.
Cloe Lacasse's first half strike helped Benfica claim a narrow 1-0 victory that leaves them three points adrift of the top two.
Having won their last six games in all competitions, Benfica will be confident that they can claim another victory over Rosengard on Wednesday.
Rosengard Women's Champions League form:
Rosengard Women's (all competitions) form:
Benfica Women Women's Champions League form:
Benfica Women (all competitions) form:
Team News
Rosengard goalkeeper Teagan Micah is expected to be shielded by the centre-back pairing of Bianca Schmidt and Gudrun Arnardottir.
Despite losing to Benfica two weeks ago, Rosengard Renee Slegers could name an unchanged lineup for the reverse fixture.
After scoring 11 league goals, Olivia Schough was Rosengaard's topscorer in the 2022 season, and she will be looking to continue her domestic form on the European stage on Wednesday.
As for the visitors, Katelin Talbert is expected to feature in goal after she dropped to the bench for the 1-0 over Rosengard.
Ana Seica is set to partner Carole Costa in central defence, and they will be looking to help Benfica record a fifth consecutive clean sheet in all competitions.
Lacasse will retain her place in the forward line after scoring in Benfica's last two Champions League games.
Rosengard possible starting lineup:
Micah; Oling, Schmidt, Arnardottir, Wik; Persson, Ayinde, Holdt; Bredgaard, Schough, Kullashi
Benfica Women possible starting lineup:
Talbert; Valeria, Ucheibe, C. Costa, Alves; Vitoria, Pauleta, Faria; Nazareth, Lacasse, Raysla