Due to the ongoing conflict in Ukraine, the second leg will take place in Kosice, Slovakia, with the Czech side firmly in control of the tie.
Match preview
Dnipro-1 found themselves 2-0 down inside 37 minutes of the first leg following a quick-fire brace from Ivan Schranz, while Vaclav Jurecka had a goal disallowed early in the second half.
Igoh Ogbu's 58th-minute red card gave the Ukrainian side a glimmer of hope, but they ultimately failed to make the most of the one-man advantage, with Conrad Wallem coming off the bench to score Slavia's third late on.
Three days later, Dnipro-1 were held to a 1-1 draw by Minai in the Ukrainian Premier League, with Domingo Blanco netting a 93rd-minute equaliser from the penalty spot following Eduard Sarapiy's sending off just after half time.
It would not be unfair to say that Oleksandr Kucher's men have struggled to find their feet in the 2023-24 season, as they have managed to win just one of their five outings across all competitions.
Having suffered a 5-3 aggregate loss to Greek club Panathinaikos in the Champions League second qualifying round, Dnipro-1 will be desperate to turn things around against Slavia Prague and secure European football.
While Dnipro-1 have experienced an inconsistent start to the new campaign, Slavia Prague have kicked off the 2023-24 season with five consecutive wins across all competitions.
Following three straight victories in the Czech Liga, Jindrich Trpisovsky's men made light work of Dnipro-1 before defeating Mlada Boleslav 1-0 on Sunday, racking up 15 shots while preventing the hosts from registering a single attempt on target.
Despite winning each of their opening four league games - scoring eight goals while conceding just twice - Slavia find themselves second in the Czech top flight behind arch-rivals Sparta Prague, who have netted 13 goals across four victories this term.
Still, the Red and Whites will be happy with their early-season efforts and confident of regaining their domestic crown, having clinched three straight Czech Liga titles between 2018-19 and 2020-21 before finishing as runners-up in each of the past two campaigns.
It goes without saying that Slavia will also be confident of booking their place in the next phase of Europa League qualification, as they already have one foot in the playoff round.
Dnipro-1 Europa League form:
L
Dnipro-1 form (all competitions):
Slavia Prague Europa League form:
W
Slavia Prague form (all competitions):
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Team News
Dnipro-1 have several key players sidelined through injury at the moment, including goalkeeper Yevgen Volynets, right-back Denys Miroshnichenko and forward Maksym Tretyakov.
Oleksandr Kucher will likely continue with a 4-2-3-1 formation, with Oleksandr Filippov leading the line, while Ruslan Babenko and Valentyn Rubchynskyi are expected to anchor the midfield.
Slavia Prague, on the other hand, will be without the injured duo of David Pech and David Doudera for the second leg, with the latter picking up a muscle injury last month.
Ivan Schranz, Mick van Buren and Mojmir Chytil have all bagged two goals and one assist in five appearances this term, while Lukas Provod has contributed one goal and three assists.
Dnipro-1 possible starting lineup:
Kinareikin; Kapliyenko, Adamyuk, Sarapiy, Kravets; Babenko, Rubchynskyi; Bill, Pikhalyonok, Lednev; Filipov
Slavia Prague possible starting lineup:
Kolar; Masopust, Ousou, Holes, Provod; Dorley, Wallem; Tomic, Sevcik, Jurecka; Schranz