The 4-0 thrashing of the Swedes was soured by the injuries sustained to the key attacking duo.
Lukaku picked up an ankle problem, while Werner suffered a hamstring injury.
Blues boss Tuchel confirmed both forwards will be sidelined in the coming weeks even though specific prognoses are yet to be determined.
Callum Hudson-Odoi laid on Kai Havertz's goal as the duo linked up well in smart cameos against Malmo, leaving manager Tuchel to lay down the gauntlet for Saturday's Norwich clash and beyond.
"There's always a chance to prove a point and it's always necessary to prove it for our players, so I would say to them, prove a point now," said the Chelsea boss.
"Everybody is invited to join the race for minutes on the pitch.
"And when they have minutes on the pitch they need to show the hunger on the pitch, to be decisive in the box.
"This is what we did against Malmo and we need exactly the same thing on Saturday against Norwich.
"We need goals to win matches and we want to find solutions without Romelu and Timo, that's clear."
Insisting he prefers to select for each match on its merits rather than for rotation's sake, Tuchel added: "We take the games step by step, there's no more important game than the game we play on this matchday.
"The guys who are on the pitch deserve to start, there are no gifts and no resting players.
"We put the best line-up in our belief to have the best chance to beat Brentford in Brentford and then we put the best line-up on this very day to beat whoever arrives in the Champions League.
"And the next one is Saturday 1230pm and we will put again the best team.
"We will have to be very careful in recovery."
It will be a chance for some more fringe players to step up in the pair's absence but how should they line-up?
Tuchel has favoured playing 3-4-3 or 3-5-2 formations so far this season and this is unlikely to change that for the visit of Norwich.
There are plenty of options at the back with Antonio Rudiger and Thiago Silva proving the most consistent while Trevoh Chalobah has impressed when he has played.
Ben Chilwell has scored in his last three Premier League appearances for Chelsea and should retain his place, while Cesar Azpilicueta is likely to line-up on the opposite side.
Jorginho scored two penalties against Malmo and looks set to be partnered with N'Golo Kante in the middle.
It is further up the pitch where changes are likely to be made. Havertz has played as a No.9 role for the Blues before and is the best option at Tuchel's disposal.
Hudson-Odoi impressed after coming off the bench against Malmo and could take up his place in the right of the attacking three.
Mason Mount would the line-up on the left to complete the attacking trio.