The 22-year-old was taken off with an apparent muscular problem in their final Bundesliga game of the season against Mainz 05 and will need checking over - even if reports have since stated that his withdrawal was precautionary.
However, Glasner will certainly be without key defensive figure Martin Hinteregger, while Danny da Costa, Diant Ramaj and Jesper Lindstrom are also sidelined for the contest.
With Hinteregger absent, Almamy Toure will take his place at the back alongside Tuta and Ndicka - assuming the latter is fit enough - alongside Kevin Trapp.
Glasner did not grant Ansgar Knauff or Filip Kostic a single minute's worth of rest at the weekend, but the two key wide men are guaranteed starters in Seville regardless.
Sebastian Rode and Djibril Sow will act as the midfield enforcers for Die Adler, with Jens Petter Hauge and Europa League top scorer Daichi Kamada just ahead of them in the final third.
Kamada could become the first-ever Japanese player to score in a European final this week, and the Frankfurt line will again be led by 11-goal striker Rafael Borre.
Frankfurt possible starting lineup: Trapp; Toure, Ndicka, Tuta; Knauff, Sow, Rode, Kostic; Kamada, Hauge; Borre