The near-neighbours last clashed 2021 and they will now meet to celebrate 150 years of their rivalry in Glasgow.
The rivals played their first-ever match against each other in 1872 and have since faced one another over 100 times.
So ahead of this special renewal of the rivalry, talkSPORT.com takes a look at the head-to-head record between the two…
England vs Scotland: Head-to-head record
England and Scotland have faced each other a total of 115 times in all competitions.
The Three Lions have won 48 of those games while the Tartan Army have won slightly less, coming out on top in 41.
READ MORE ON ENGLAND
Harvey Barnes considering Scotland switch after England snub despite Three Lions cap
Maguire's 'top' display and Southgate too defensive - What we learnt from England draw
There have also been 26 draws between the two with the most recent coming at Euro 2020.
In fact, that 0-0 draw at Wembley during the Euro 2020 group stage was the last time England played against Scotland.
The fixture has been a rarity in recent years but that wasn't always the case with an annual meeting taking place from 1884 all the way to 1989.
Scotland were the more dominant side back then, winning a large amount of matches between the two before England began to take over.
Most read in Football
Rubiales finally resigns, Antony takes Man United leave of absence, Sancho showdown talks
Chelsea 'fear' Romeo Lavia could be out until November following training ground injury
Rice says Arteta made him feel like Will from The Inbetweeners on first day at Arsenal
Piers Morgan reveals all about 'cloak and dagger' interview with shamed Luis Rubiales
Unfortunately, those annual clashes came to an end 33 years ago and since then the teams have only met eight times.
And there have only been five clashes over the last ten years.
England are unbeaten in those games but Scotland have managed to earn a draw in the most recent meetings between the two.
England vs Scotland: talkSPORT coverage
The 150th-anniversary match is scheduled to kick off at 7:45pm on Tuesday, September 12.
It will be played at Hampden Park in Glasgow.
talkSPORT will have live coverage of the game presented by Adrian Durham.
Commentary comes from Jim Proudfoot, former Scotland striker Chris Iwelumo and former England defender Stuart Pearce
The game will also be available to watch for free on Channel 4.
To tune in to talkSPORT or talkSPORT 2 through the website, click HERE for the live stream.
You can also listen via the talkSPORT app, on DAB digital radio, through your smart speaker and on 1089 or 1053 AM.