The Young Lions haven't won this competition since 1984 but boast a strong squad packed with Premier League talent.
Lee Carsley's men start the tournament as one of the favourites, along with Spain and France.
Stars from Aston Villa, Arsenal, Manchester City, Chelsea and Newcastle have all been included as England look to launch a strong challenge.
And they open up with a tie against a side that consists of mainly players based in the Czech league, barring Vitezslav Jaros, who plays for Stockport County.
England U21s vs Czech Republic U21s: talkSPORT coverage
This Euro Under-21 Group C game will take place on Thursday, June 22.
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Kick-off at the Batumi Arena in Georgia is set for 5pm UK time.
talkSPORT will have live commentary from Olly Clink and former England striker David Nugent.
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England U21s vs Czech Republic U21s: Team news
Djed Spence would have been included in Carsley's squad but misses out due to a knee injury.
But Emile Smith Rowe has done enough to overcome his own injury problems to feature.
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England U21 Squad in full
Goalkeepers: Josh Griffiths (West Bromwich Albion), Carl Rushworth (Brighton and Hove Albion), James Trafford (Manchester City)
Defenders: Max Aarons (Norwich City), Jarrad Branthwaite (Everton), Levi Colwill (Chelsea), Charlie Cresswell (Leeds United), Taylor Harwood-Bellis (Manchester City), Ben Johnson (West Ham United), Luke Thomas (Leicester City)
Midfielders: Tommy Doyle (Manchester City), Harvey Elliott (Liverpool), James Garner (Everton), Angel Gomes (Lille), Curtis Jones (Liverpool), Jacob Ramsey (Aston Villa), Oliver Skipp (Tottenham Hotspur)
Forwards: Cameron Archer (Aston Villa), Morgan Gibbs-White (Nottingham Forest), Anthony Gordon (Newcastle United), Noni Madueke (Chelsea), Cole Palmer (Manchester City) Emile Smith Rowe (Arsenal)
England U21s vs Czech Republic U21s: What has been said?
Aston Villa's Jacob Ramsey believes this squad's attitude is very different to previous young England sides.
Speaking to the press, he said: "One thing that I will say about this group - when we've looked at previous groups before, and previous camps, there's probably a different mentality.
"I think, before, people have put us down as big favourites and that we've gone there to win it.
"But in this group we've got a bit of a humble side. I think everyone knows how well we can play together, and we probably are one of the favourites.
"We will go into this tournament respecting every team. For my personal opinion in this group, I feel like we've got the right balance and, hopefully, when we go out there we will show that."