The Brewers are hovering just above the relegation zone, while the Robins are five points adrift in 22nd position.
Match preview
Burton went into Saturday's home clash with Stevenage off the back of a woeful run of form which saw them take just two points from 10 matches.
However, the Brewers managed to halt their slide with a 2-1 victory at the Lamex Stadium thanks to goals from Mark Helm and Tom Hamer.
That result moved Burton three points clear of the drop zone with just three matches left to play in the League One season.
Martin Paterson's side will now return to the Pirelli Stadium for their final two home games against Cheltenham and Reading, before they wrap up the campaign with an away game against Fleetwood Town.
The Brewers have struggled in recent home outings, having lost each of their last six matches in front of their own supporters.
Meanwhile, Cheltenham are languishing in 22nd position after losing four consecutive games since beating Fleetwood at the end of March.
They shipped three goals in Saturday's home clash with Bristol Rovers before Liam Sercombe netted a consolation in the 94th minute.
The Robins are now five points adrift of the Brewers, and while they may have a game in hand, they know they need to win on Tuesday to have a realistic chance of beating the drop.
However, Cheltenham's hopes of claiming all three points will be tempered by the fact they have failed to win any of their 10 meetings with Burton since recording a 1-0 home win in November 2012.
The visitors have also lost three of their last four away matches against the Brewers (D1) since claiming their most recent win at the Pirelli Stadium in October 2011.
Burton Albion League One form:
L
L
D
L
L
W
Cheltenham Town League One form:
D
W
L
L
L
L
Team News
Burton captain John Brayford is a doubt after he was forced off in the first half of Saturday's win over Stevenage.
Mason Bennett was withdrawn at half time with a fitness issue and will have to be assessed ahead of Tuesday's contest.
The Brewers will hope to have Ryan Sweeney available after the defender missed the weekend's game due to personal reasons.
As for Cheltenham, they remain without the services of forward Rob Street, while defender Lewis Freestone is a doubt after missing the last two matches.
Tom Pett was forced off in the opening 20 minutes against Bristol Rovers, making him an obvious fitness concern for Tuesday's away trip.
Sercombe could be recalled to the starting lineup after coming off the bench to score a late goal in Saturday's defeat.
Burton Albion possible starting lineup:
Crocombe; Moon, Nsiala, Hughes; Hamer, Harper, Oshilaja, Seddon; Powell, Helm, Hugill
Cheltenham Town possible starting lineup:
Southwood; Smith, Davies, Shepherd; Long, Kinsella, Harrop, Bonds, Ferry; Taylor, Nuttall