Xavi's side will enter the contest off the back of a 2-0 victory over basement side Almeria on Thursday.
The Catalan outfit are now four points ahead of third-placed Girona in the table, so they would secure the position behind champions Real Madrid with a win in this contest.
Here, Sports Mole rounds up Barcelona's latest injury and suspension news ahead of the clash with Rayo, who are 15th in the division, six points ahead of 18th-placed Cadiz.
Gavi
Status: Out
Type of injury: Knee
Possible return date: Unknown
Gavi suffered a serious knee injury while representing Spain towards the end of 2023, and the highly-rated midfielder will not be available for selection until next season.
Alejandro Balde
Status: Out
Type of injury: Hamstring
Possible return date: Unknown
First-choice left-back Balde underwent a hamstring operation at the end of January, and the Spain international will be out until next season.
Frenkie de Jong
Status: Out
Type of injury: Ankle
Possible return date: Unknown
De Jong suffered another ankle injury during the La Liga clash with Real Madrid on April 21, and the midfielder is expected to be miss the remainder of the season. However, he has been named in the provisional Netherlands squad for Euro 2024.
Ronald Araujo
Status: Major doubt
Type of injury: Knee
Possible return date: May 19 (vs. Rayo)
Araujo is currently suffering with pain in his knee, and it has been claimed that he could miss the remainder of the season, but the centre-back, as it stands, has not been officially ruled out of this match.
BARCELONA'S SUSPENSION LIST
Barcelona have no players suspended for this match, and Ilkay Gundogan will be available again after missing the clash with Almeria last time out.