The Gunners have been enjoying their pre-season so far, having began their visit to America with a comfortable 2-0 win over Everton.
Orlando City drew to Atlanta United 1-1 on Sunday but will now take a one-match break from MLS action to host Arsenal in the FC Series.
The Gunners are out to secure a third successive pre-season win with new signing Gabriel Jesus scoring three goals in his first two games.
Arsenal will then be involved in a London derby with Chelsea in Florida before returning to the Emirates to finish their pre-season games.
Arsenal vs Orlando City: Date and start time
This pre-season friendly will take place on Thursday, July 21.
Kick-off at the 25,500-capacity Exploria Stadium in Orlando will take place at 12.30am UK time.
Arsenal vs Orlando City: TV channel and live stream
This game will once again be available to watch live on Arsenal.com and the Arsenal app.
The Gunners are streaming every game on their club website with match passes costing £5.99 each. For more information, click HERE.
talkSPORT will have regular updates from Orlando while talkSPORT.com will have all the reaction from the game later that morning.
To tune in to talkSPORT or talkSPORT 2 through the website, click HERE for the live stream. You can also listen via the talkSPORT app, on DAB digital radio, through your smart speaker and on 1089 or 1053 AM.
Arsenal vs Orlando City: Team news
Mikel Arteta has revealed that he could have as many as FIVE players missing for the end of the club's tour of the USA.
Speaking after the win over Everton, Arteta said: "We have Kieran [Tierney] with a slight problem, we have Emile [Smith Rowe] with a muscular niggle, Tomiyasu as well & Ben [White].
"Vieira has a slight problem in his bone, hopefully he will start to train in the next week or so."
As for Orlando, goalkeeper Mason Stajduhar is out with a jaw injury and he'll join ACL victim Gaston Gonzalez is being unavailable for this one.
Arsenal vs Orlando City: What has been said?
Gabby Agbonlahor believes that Arsenal still have work to do if they're to challenge for a top four finish this term.
Speaking on talkSPORT, the former Aston Villa striker said: "For Arsenal it's a big statement signing Gabriel Jesus, but still, they need more work.
"There's talk of Zinchenko, who I think will be a great signing, a great addition. He's versatile, but maybe they need another right-sided winger as back up to Bukayo Saka because Nicolas Pepe has not done it.
"These teams know that they need to keep improving their squad because it's going to get harder and harder to challenge for the top four.
"Arsenal don't want to be cut adrift. They want to bring in top, top players because Spurs are doing the same.
"Spurs have had a great window so it's going to be even harder this season to get that top four and Man United fans, once again, will say that I don't think they've got much chance to get top four again this season."