Despite battling their way to a 2-0 win over Senegal earlier this week, Van Gaal has freshened up his side with the inclusion of Jurrien Timber, Teun Koopmeiners and Davy Klaassen.
Matthijs de Ligt, Steven Berghuis and Vincent Janssen are the trio to drop out of the team, with Cody Gakpo being used alongside Steven Bergwijn in attack.
Despite making his return from injury in the first game from the substitutes' bench, Memphis Depay remains among the replacements.
Meanwhile, Enner Valencia has shaken off a knee issue to start for Ecuador as he bids to make World Cup history for the South American nation.
Gustavo Alfaro has made just one alteration from the team which started the win over Qatar, with Jackson Porozo replacing Romario Ibarra.
Netherlands: Noppert; Timber, Van Dijk, Ake; Dumfries, Koopmeiners, De Jong, Blind; Klaassen; Gakpo, Bergwijn
Ecuador: Galindez; Preciado, Porozo, Torres, Hincapie, Estupinan; Plata, Mendez, Caicedo, E. Valencia; Estrada
Armenia 
Faroe Islands 
Malta 
Liechtenstein 
Belgium 
Italy 
France 
Israel 
Greece 
England 
Rep. Of Ireland 
Finland 
Slovenia 
Norway 
North Macedonia 
Latvia 
Russia 
Brunei 
Cyprus 
Lithuania 
Portugal 
Poland 
San Marino 
Gibraltar 
Scotland 
Croatia 
Romania 
Kosovo 
Luxembourg 
Bulgaria 
Northern Ireland 
Belarus 
Denmark 
Spain 
Switzerland 
Serbia 
Azerbaijan 
Estonia 
Georgia 
Ukraine 
Andorra 
Moldova 
Montenegro 
Iceland 
Turkey 
Wales 
Albania 
Czech Republic 
Sweden 
Slovakia 
Germany 
Bosnia & Herzegovina 
Netherlands 
Hungary 
Bordeaux