Aurelien Tchouameni suffered an ankle injury during Tuesday's 3-1 defeat to AC Milan in the Champions League, with the France international expected to be on the sidelines for the next month.
Eduardo Camavinga is likely to be the player to benefit in terms of the starting side, while Rodrygo, who came off the bench against Milan after recovering form an injury, is also set to start against Osasuna.
There is still a slight doubt regarding Federico Valverde, as the Uruguay international was replaced at the interval of the European contest last time out due to a back problem.
However, Valverde is expected to be cleared to feature in the middle of midfield.
Tchouameni has joined Dani Carvajal, David Alaba and Thibaut Courtois are on the sidelines, meaning that four first-team players are currently unavailable for selection.
Jude Bellingham is still waiting for his first goal of the campaign, and the England international is expected to be used in a deeper midfield role against Osasuna.
Kylian Mbappe has scored six La Liga goals this season, but he is still struggling to find his rhythm, and Ancelotti will be hoping that the 25-year-old can inspire the team to all three points this weekend.
Real Madrid possible starting lineup: Lunin; Vazquez, Militao, Rudiger, Mendy; Valverde, Camavinga, Bellingham; Rodrygo, Mbappe, Vinicius