Los Blancos will be confirmed as champions on Saturday if they overcome Cadiz and then second-placed Barcelona fail to beat third-placed Girona later in the afternoon.
Ancelotti is expected to make wholesale changes to his XI this weekend, as the Spanish giants will face Bayern Munich in the second leg of their Champions League semi-final next week, with the tie level at 2-2.
Thibaut Courtois will make his first appearance of the season, with the Belgian goalkeeper finally ready to feature after two knee operations during the 2023-24 campaign.
David Alaba is Real Madrid's only injured player
Ancelotti has also confirmed that there will be starts for Eder Militao and Arda Guler, while the likes of Brahim Diaz, Joselu, Fran Garcia and Dani Ceballos should also be in the XI.
David Alaba is Real Madrid's only absentee at this moment in time, with the Austria international out until next season due to an ACL injury which he suffered in December.
There had been some concern over the fitness of Aurelien Tchouameni, with the midfielder said to be struggling with a foot injury, but the Frenchman has been named in the squad.
Jude Bellingham is also in the squad, and Ancelotti has backed the England international to find his best form again after struggling against Bayern last time out following an illness.
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"Bellingham simply had an intestinal virus that did not give him the chance to recover 100% against Bayern. Now he is very well, he will do very well as he always has," said the head coach during his press conference.
Bellingham is unlikely to be a starter against Cadiz, though, with the England international expected to be rested alongside the likes of Toni Kroos, Rodrygo, Federico Valverde and Vinicius Junior.
"The objective is very clear: add three points to get closer to winning this competition. We only think about fulfilling our objective against a team that is fighting to avoid relegation, that arrives at the Bernabeu with desire and enthusiasm," added Ancelotti during his press conference.
Dani Carvajal is also back in the squad after missing out against Bayern through suspension, and the Spain international could start on Saturday despite the fact that he will be in the XI for the second leg of the Champions League semi-final next week.
Real Madrid squad vs. Cadiz:
Courtois, Lunin, Kepa; Carvajal, Militao, Nacho, Vazquez, F Garcia, Rudiger, Mendy; Bellingham, Kroos, Modric, Camavinga, Valverde, Tchouameni, Ceballos, Guler; Vinicius, Rodrygo, Joselu, Brahim