Disappointing results last season prompted the appointment of Marco Baroni, while Venezia are also under new management despite winning the Serie B playoffs.
Match preview
Not only did Maurizio Sarri quit in mid-season after several weeks of airing various frustrations, but his replacement as Lazio head coach, Igor Tudor, later followed him through the exit door at Stadio Olimpico.
Now, Marco Baroni starts the process of reinvigorating a squad that finished runners-up to Napoli in 2023 but stumbled to a seventh-placed finish last term.
The ex-Hellas Verona coach was not exactly Lazio fans' first-choice appointment, but despite their protests, he will lead the Rome club into the post-Ciro Immobile era.
Following a year ruined by failing fitness and form, the Biancocelesti's talisman was left out of Luciano Spalletti's Italy squad for Euro 2024, and having subsequently left for Turkish giants Besiktas, new impetus will be sought in the final third.
After Sergej Milinkovic-Savic departed for Saudi Arabia last summer, big earners such as Luis Alberto and Felipe Anderson have now followed Immobile, helping to balance the books and fund moves for Tijjani Noslin and Loum Tchaouna.
During a packed pre-season schedule, Lazio won four times and drew twice, finishing off with a 1-0 victory over Cadiz, so they should be primed to meet newly promoted opponents.
Sprinting to the finish line with seven wins from their last 10 matches, Venezia booked a return to Serie A after two years away, coming out on top in Italy's second-tier playoffs.
After narrowly missing out to Como for automatic promotion, they beat Palermo in the semi-finals, before Christian Gytkjaer scored the decisive goal in a two-legged decider against Cremonese.
However, head coach Paolo Vanoli then took up an offer to fill the managerial vacancy at Torino, so Eusebio Di Francesco was hired to lead the Arancioneroverdi's upcoming battle for survival.
After unsuccessful spells at Cagliari and Hellas Verona, Di Francesco almost kept Frosinone up last season, but now the ex-Roma midfielder and manager starts a new Serie A season back at Stadio Olimpico.
Four wins from six in pre-season suggested his Venezia side were in good shape for last Sunday's Coppa Italia tie at Stadio Rigamonti, where the Lagunari played their first competitive contest since earning promotion.
They suffered a 3-1 loss to Serie B side Brescia, though, striking a blow to morale just before their testing trip to the capital.
Lazio pre-season form:
W
D
W
W
D
W
Venezia form (all competitions):
Team News
Summing up a fairly dismal season, Ciro Immobile was still Lazio's Serie A top scorer last term despite scoring just seven goals, but the hosts' front line will now be led by Taty Castellanos.
The latter only found the net four times during his first year in Italy, but he should link up with new club captain Mattia Zaccagni in the final third.
New boys Loum Tchaouna and Tijjani Noslin are also vying for selection, while Matteo Guendouzi starts in midfield after making his loan move from Marseille permanent.
While Lazio struggled for a regular scorer in 2023-24 - even turning to goalkeeper Ivan Provedel to save them at the end of a Champions League clash with Atletico Madrid - Venezia's Joel Pohjanpalo racked up 22 goals in Serie B, tying for top spot in the scoring charts.
However, the Finnish striker is sidelined by a thigh problem, so Nicholas Pierini and Christian Gytkjaer may be paired up front on Sunday evening.
American midfielder Tanner Tessmann has been heavily linked to both Fiorentina and Inter Milan so was not involved last weekend, while his compatriot Gianluca Busio sustained an injury during the USA's Olympics campaign and will be out of action until September.
Nonetheless, new signings Alfred Duncan and Gaetano Oristanio - both of whom previously spent time on loan at Cagliari - are available.
Lazio possible starting lineup:
Provedel; Lazzari, Gila, Romagnoli, Pellegrini; Vecino, Guendouzi; Tchaouna, Noslin, Zaccagni; Castellanos
Venezia possible starting lineup:
Joronen; Altare, Svoboda, Sverko; Sagrado, Duncan, Crnigoj, Zampano; Oristanio, Pierini; Gytkjaer