As a result, Rodri will again feature in a holding midfield position, but there could be a change from Monday's goalless draw with Sweden, with Thiago Alcantara potentially replacing Pedri.
Alvaro Morata's involvement is also a topic of debate, with the forward missing a huge opportunity against Sweden, but head coach Luis Enrique is expected to keep faith with the 28-year-old.
There are unlikely to be any changes to the back four, but there are a number of options in the forward positions, with Pablo Sarabia, Mikel Oyarzabal and Gerard Moreno pushing for spots in the side.
Spain could also look to utilise Adama Traore's pace against a wounded Poland, but Enrique may decide to ultimately keep faith with Dani Olmo and Ferran Torres in the wide areas.
Aymeric Laporte and Pau Torres appear to be the team's two first-choice centre-backs at the European Championship, while Marcos Llorente is in charge of the right-back spot.
Enrique is certainly not short of options or quality, but the overall performance against Sweden was strong despite the relatively disappointing nature of the result.
Spain possible starting lineup: Simon; M Llorente, Laporte, P Torres, Alba; Koke, Rodri, Thiago; F Torres, Morata, Olmo