The City boss was in an animated mood for the crunch Premier League clash - a match that was heavily billed as a decider in the title race.
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Emotions were clearly running high for both sides and that was clear when Kevin De Bruyne appeared to be brought down in the penalty area by Thomas Partey.
Despite widespread appeals from the City team, the referee opted to instead give Arsenal a free-kick, much to Guardiola's annoyance.
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However, it was the Spaniard's behaviour after a seemingly clear foul from Bernardo Silva on Granit Xhaka which caught our pundits' attention.
Describing the action on talkSPORT, Sam Matterface said: "Michael Oliver, he initially said play on and then he decided that he was going to pull the whistle out of his pocket and blow it.
"Pep Guardiola is going absolutely crazy, he is losing his head. He's almost on the pitch, he's being restrained by Craig Pawson. He's going absolutely mad."
Pearce reacted: "I don't know what Pep is doing down there.
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"He's striking out. He's pushed the fourth official away from him. He really needs to calm down a little bit."
He added: "Only he knows if he's out of control or not. I've never seen him as over-the-top as this.
"I've seen other managers but from his point of view I think he gets caught up in the game."
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Former Arsenal winger Perry Groves then chimed in, saying: "Can someone tell him that his team are 1-0 up at the moment?"
Thankfully for Guardiola, his frustrations came after City has already taken the lead Kevin De Bruyne.
However the head coach will no doubt have something to say if that penalty appeal proves to be decisive in the outcome of the clash.