The Reds picked up where they left off in the competition with a 5-1 thumping of West Ham United in the third round last month, the same scoreline they crushed the Irons by in the 2023-24 quarter-finals.
Arne Slot's men missed the chance to go back to the top of the Premier League rankings on Sunday, though, playing out a gripping 2-2 draw with Arsenal at the Emirates as the Dutchman lost his 100% away record.
Here, Sports Mole rounds up all of Liverpool's latest injury and suspension news ahead of their clash with Brighton, who bested Wolverhampton Wanderers 3-2 in round three of the EFL Cup.
Diogo Jota
Status: Out
Type of injury: Abdominal
Possible return date: Unknown
Diogo Jota was unavailable for the trip to Arsenal due to the abdominal injury he suffered against Chelsea two weekends ago - it is still unclear how severe his issue is, but he will certainly not be a part of the squad on Wednesday.
Alisson Becker
Status: Out
Type of injury: Hamstring
Possible return date: November 24 (vs. Southampton)
Alisson Becker is making positive strides in his recovery from his latest hamstring injury, but Liverpool will have to make do without their number one goalkeeper for at least another few weeks.
Federico Chiesa
Status: Major doubt
Type of injury: Fitness
Possible return date: October 30 (vs. Brighton)
Federico Chiesa is being given more time to hit peak fitness and acclimatise to the English game, but having not made the squad against Arsenal, the Italy international remains a serious doubt for the midweek affair.
Harvey Elliott
Status: Out
Type of injury: Foot
Possible return date: November 9 (vs. Aston Villa)
Harvey Elliott should now be in the second half of his recovery from September's foot fracture, but the midfielder likely has another couple of weeks of rehabilitation to go before he will be back in the first-team picture.
Conor Bradley
Status: Major doubt
Type of injury: Unspecified
Possible return date: October 30 (vs. Brighton)
Conor Bradley has been missing from Liverpool's recent showdowns with Arsenal, Chelsea and RB Leipzig with an unspecified concern, and a question mark is also hanging over his head for the trip to Brighton.
LIVERPOOL'S SUSPENSION LIST
Liverpool have no players suspended for this match.