The Catalan giants played out a goalless draw with Getafe last weekend, which has left them in second position in the table on 73 points.
Xavi's side have already secured the runners-up spot in the division behind Real Madrid, and they will be looking to record their 22nd league victory of the campaign against the Yellow Submarine.
Here, Sports Mole rounds up all of the team's latest injury and suspension news ahead of the fixture with Villarreal, who are battling to keep hold of seventh spot in the division.
Gerard Pique
Status: Major doubt
Type of injury: Groin
Possible return date: May 22 (vs. Villarreal)
Gerard Pique faces a battle to return on Sunday due to a thigh problem, but he could potentially be in the squad against Villarreal depending on how he recovers over the next couple of days.
Sergi Roberto
Status: Out
Type of injury: Muscle
Possible return date: Unknown
Sergi Roberto underwent surgery on a thigh problem towards the end of last year; the Spaniard is now back in first-team training but is targeting a return for pre-season.
Pedri
Status: Out
Type of injury: Hamstring
Possible return date: Unknown
Spanish midfielder Pedri will again be absent against Villarreal due to a hamstring problem.
Sergino Dest
Status: Major doubt
Type of injury: Muscle
Possible return date: May 22 (vs. Villarreal)
Sergino Dest picked up a muscular problem during Barcelona's 1-0 defeat to Rayo Vallecano on April 24, and the right-back faces an uphill task to prove his fitness for this match.
Nico Gonzalez
Status: Out
Type of injury: Toe
Possible return date: Unknown
Barcelona recently revealed that Nico Gonzalez has fractured his toe, and the injury will keep the midfielder out until pre-season.
Eric Garcia
Status: Out
Type of injury: Hand
Possible return date: Unknown
Eric Garcia recently underwent an operation on his injured thumb, and the central defender will be absent for the team's final match of the season with Villarreal.
Memphis Depay
Status: Major doubt
Type of injury: Hip
Possible return date: May 22 (vs. Villarreal)
Memphis Depay is a major doubt for this weekend's clash with the Yellow Submarine, having picked up a hip injury in the goalless draw with Getafe last time out.
BARCELONA'S SUSPENSION LIST
Barcelona have no suspension issues heading into this match, with Jordi Alba and Frenkie de Jong both available again after being banned against Getafe.