The Algeria international was taken off with a knock in the closing stages of Saturday's 1-0 win at Bournemouth, having previously been the victim of a red card-worthy tackle from Ilya Zabarnyi.
Ait-Nouri's status for Tuesday is still up in the air, as is Rodrigo Gomes's due to his own unspecified injury, so Vitor Pereira might have to call upon 18-year-old Pedro Lima to fill the void on the left.
It should otherwise be as you were for the hosts after their third win from four games in all competitions, although centre-forward Jorgen Strand Larsen is now back from a hamstring injury to offer a focal point up front.
All of Jean-Ricner Bellegarde, Marshall Munetsi and match-winner Matheus Cunha justified their selections at the weekend, though, so Pereira may see no need to break up the trio just yet.
Further back, Joao Gomes's half-time substitution at the Vitality Stadium was seemingly tactical after he was booked towards the end of the first half, so the Brazilian should join Andre, Lima and Nelson Semedo in the bank of four once more.
If Emmanuel Agbadou is unable to shake off his own concern, Santiago Bueno, Toti Gomes and Matt Doherty should all reprise their defensive roles in front of Jose Sa.
Sasa Kalajdzic (knee), Yerson Mosquera (knee), Hwang Hee-chan (hamstring), Leon Chiwone (knee) and Enso Gonzalez (knee) will play no part in Tuesday's game.
Wolverhampton Wanderers possible starting lineup:
Sa; Doherty, Toti, Bueno; Semedo, Gomes, Andre, Lima; Bellegarde, Munetsi; Cunha
Written by
Ben Knapton