The 20-year-old has a good chance of being promoted to the starting lineup in the Spanish capital, where if he makes the net bulge, he will become the youngest Englishman to ever score a UCL goal against Los Blancos.
Gittens replaced Pascal Gross at half time in the weekend's 2-1 Bundesliga win over St Pauli after the ex-Brighton & Hove Albion man sustained a knock, one that renders him doubtful for Tuesday's rematch of the 2023-24 final.
As a result, Marcel Sabitzer could revert to a midfield role alongside captain Emre Can, as Gittens joins Donyell Malen, Julian Brandt and Serhou Guirassy in Dortmund's offensive quartet.
Karim Adeyemi would normally be among the attacking starters, but the German is out for several weeks with the hamstring injury he suffered in the 7-1 thrashing of Celtic, where he netted a hat-trick.
Further back, Niklas Sule is also uncertain owing to illness, so Waldemar Anton and Nico Schlotterbeck should be tasked with shielding number one goalkeeper Gregor Kobel.
Yan Couto's muscular injury means that Julian Ryerson will have the daunting task of trying to keep Vinicius Junior quiet, and Ramy Bensebaini will be charging up and down the left flank for BVB.
Borussia Dortmund possible starting lineup:
Kobel; Ryerson, Anton, Schlotterbeck, Bensebaini; Can, Sabitzer; Malen, Brandt, Gittens; Guirassy