The Catalan giants, who are currently top of the La Liga table, will enter this weekend's affair off the back of a 4-1 victory over Bayern Munich in the Champions League, with Raphinha hitting a hat trick.
Frenkie de Jong, Gavi and Dani Olmo are all back from recent injury problems, meaning that Flick has a big decision to make when it comes to the trio, but they could again be used off the bench.
Marc Casado has been praised by his manager for his form this season, and the Spaniard is again likely to feature in the middle of midfield alongside Pedri, with Fermin Lopez playing as the number 10.
Fermin provided two assists in an outstanding performance against Bayern, while there will be no surprises ahead of him, with Lamine Yamal, Raphinha and Robert Lewandowski all in line to start.
Lewandowski has scored 15 times in 13 appearances in all competitions during the 2024-25 campaign, including 12 goals in 10 league outings, and the Poland international will be looking to score his first La Liga goal against Real Madrid on Saturday.
Inaki Pena is set to continue between the sticks, while there are unlikely to be any surprises in the defensive areas.
Ronald Araujo, Ferran Torres, Andreas Christensen, Marc Bernal and Marc-Andre ter Stegen are all on the sidelines through injury for Barcelona, but there is a chance that Eric Garcia could be back in the squad.
Barcelona possible starting lineup: Pena; Kounde, Cubarsi, I Martinez, Balde; Casado, Pedri; Yamal, Fermin, Raphinha; Lewandowski