Giovanni Gonzalez scored the only goal of the match to hand Mallorca a one-goal success over Las Palmas, with the result moving the Copa del Rey finalists onto 35 points from 35 matches.
Mallorca are now 15th in the table, with Rayo Vallecano dropping to 17th, but the capital outfit are on 34 points, while Granada sit 19th on 21 points with just four games left.
Granada's relegation to the Segunda Division has therefore been confirmed ahead of their league clash with the champions Real Madrid on Saturday evening.
A disappointing campaign has seen the Andalusian outfit win just four of their 34 matches, suffering 21 defeats in the process, and they can now only pick up a maximum of 12 points more this season.
Granada are relegated ahead of Real Madrid clash
Granada have joined Almeria in being relegated to the Segunda Division for the 2024-25 campaign, and Cadiz are also facing demotion to the second tier for next season.
Cadiz are 18th in the table, eight points behind 17th-placed Rayo, with the Yellow Submarine therefore having plenty of work to do in order to stay in the division.
Granada won last season's Segunda Division to secure promotion to the top tier, but they have been unable to make it back-to-back campaigns in La Liga.
The team finished seventh and ninth respectively in the 2019-20 and 2020-21 campaigns, but they were then 18th in 2021-22 to drop down to the Segunda Division.
Granada were Segunda Division champions last season
Granada will now be playing for pride in their final four league matches of the season against Real Madrid, Rayo, Celta Vigo and Girona, but they can still have a big say in what happens at both ends of the table.
Jose Ramon Sandoval's side managed to pick up seven points from their three games against Alaves, Athletic Bilbao and Osasuna between April 14 and April 28, but the 3-0 loss at Sevilla last time out proved to be damaging in terms of their survival bid.
There is expected to be a huge turnaround this summer in terms of players, while a decision also needs to be made on Sandoval, who became Granada's third manager of the season after Paco Lopez and Alexander Medina in the middle of March.
Sandoval has a record of two wins, one draw and three defeats from his six matches at the helm.
Poor away form has ultimately cost Granada this season, with the team picking up just three points from their 17 matches on their travels, which is the worst record in the division.