Radu Dragusin is suspended after being sent off in the seventh-minute of Spurs' Europa League opener against Qarabag last week (3-0 win), while Richarlison (muscle) and Wilson Odobert (hamstring) remain sidelined with injuries.
Destiny Udogie was taken off at half time in Tottenham's 3-0 Premier League win at Manchester United on Sunday with a thigh problem, while Son Heung-min missed out entirely due to a hamstring issue, and both players are doubtful for Thursday's contest.
Ange Postecoglou will consider making some changes to his starting lineup, with Archie Gray potentially being recalled at right-back at the expense of Pedro Porro, while Ben Davies is set to start at left-back regardless as to whether Udogie is deemed fit to feature.
Cristian Romero and Micky van de Ven - the latter of whom produced a barnstorming run through Man United's midfield to provide an assist for Tottenham's opening goal last weekend - are the only two first-team centre-back options available to Postecoglou and are therefore set to continue in the first XI.
Yves Bissouma, Pape Matar Sarr and Lucas Bergvall will all be pushing for starts in midfield, with Dejan Kulusevski, Rodrigo Bentancur and James Maddison potentially given a rest, while Brennan Johnson - who has scored in each of his last four appearances - Timo Werner and Mikey Moore are all set to battle for starts in attack with Dominic Solanke.
Tottenham Hotspur possible starting lineup: Vicario; Gray, Romero, Van de Ven, Davies; Sarr, Bissouma, Bergvall; Johnson, Solanke, Werner