The Argentina international made his return from a calf injury as a substitute in the team's 3-2 victory over Newcastle United on Wednesday night.
Martinez has now been brought into the Man United XI for their final match of the 2023-24 Premier League campaign, replacing Jonny Evans in the middle of the defence.
Casemiro will again start at centre-back, but Raphael Varane is back on the bench and could be introduced for his final league appearance for the club.
Amad Diallo scored against Newcastle last time out and retains his spot in the final third, while Scott McTominay is again set to feature as the false nine for the Red Devils.
A strong bench includes Marcus Rashford, Rasmus Hojlund, Antony and Christian Eriksen, but Victor Lindelof misses out despite returning to training at the end of last week.
As for Brighton, departing head coach Roberto De Zerbi has made six changes to the side that started the 2-1 loss to Chelsea last time out.
Adam Lallana starts on his final appearance for the club, while Jason Steele comes back in between the sticks.
The home side's major absentee is Lewis Dunk, with the centre-back missing out against the 20-time English champions at the Amex due to a knee injury.
Brighton & Hove Albion: Steele; Gross, Webster, Igor, Barco; Baleba, Moder; Adingra, Lallana, Pedro; Welbeck
Subs: Baker-Boaitey, Buonanotte, Enciso, Fati, Gilmour, Lamptey, Offiah, Peupion, Verbruggen
Manchester United: Onana; Wan-Bissaka, Casemiro, Martinez, Dalot; Amrabat, Mainoo; Amad, Fernandes, Garnacho; McTominay
Subs: Bayindir, Evans, Kambwala, Varane, Collyer, Eriksen, Antony, Hojlund, Rashford