The Getafe boss may be unable to call upon Nabil Aberdin and Alvaro Rodriguez, who are struggling with muscle and ankle injuries.
David Soria will line up between the posts, and he is likely to be shielded by a back five who will be tasked with thwarting a Barcelona side that has scored 16 goals in their last three league games.
Djene, Juan Berrocal and Omar Alderete are set to operate as the three central defenders, while Juan Iglesias and Diego Rico will line up as the right and left wing-backs respectively.
Christantus Uche, who has been one of Getafe's three goalscorers this season, is expected to feature in the middle of the park alongside Luis Milla.
Alex Sola is pushing for a starting spot after featuring as a half-time substitute in Sunday's 1-1 draw against local rivals Leganes.
Carles Perez may also be considered for a starting spot, having had to settle for substitute appearances in the last two matches.
Getafe are the joint-second lowest scorers in La Liga this term, but they will hope that Mayoral's recent return from injury can improve their attacking threat.
Mayoral has come off the bench in the last two matches, and he is now in contention to make his first start since returning from injury after scoring the crucial equaliser in Sunday's derby.
Getafe possible starting lineup: Soria; Iglesias, Djene, Berrocal, Alderete, Rico; Perez, Uche, Milla, Sola; Mayoral