Gareth Southgate's men currently lead the group and just need to avoid defeat in this one to confirm their place in the knockout phase of football's biggest competition.
Robert Page's men, meanwhile, must emerge victorious to stand any chance of qualifying from what remains a finely poised Group B heading into the third round of matches.
Here, Sports Mole looks at how the squads of Wales and England weigh up against each other ahead of Tuesday's contest.
WALES
Most valuable player:
Ben Davies (£17.1m)
Overall squad value:
£137.3m
Most capped player:
Gareth Bale (110)
Leading goalscorer:
Gareth Bale (41)
Total caps:
1,020
Total goals:
93
Oldest player:
Wayne Hennessey (35)
Youngest player:
Rubin Colwill (20)
Average age:
26.4
SQUAD IN FULL
Goalkeepers: Wayne Hennessey (Nottingham Forest), Danny Ward (Leicester City), Adam Davies (Stoke City)
Defenders: Neco Williams (Nottingham Forest), Ben Davies (Tottenham Hotspur), Ben Cabango (Swansea City), Joe Rodon (Rennes, on loan from Tottenham Hotspur), Chris Mepham (Bournemouth), Ethan Ampadu (Spezia, on loan from Chelsea), Chris Gunter (AFC Wimbledon), Connor Roberts (Burnley), Tom Lockyer (Luton Town)
Midfielders: Aaron Ramsey (Nice), Joe Allen (Swansea City), Harry Wilson (Fulham), Joe Morrell (Portsmouth), Dylan Levitt (Dundee United), Rubin Colwill (Cardiff City), Jonny Williams (Swindon Town), Matthew Smith (Milton Keynes Dons), Sorba Thomas (Huddersfield Town)
Forwards: Gareth Bale (Los Angeles FC), Dan James (Fulham, on loan from Leeds United), Kieffer Moore (Bournemouth), Mark Harris (Cardiff City), Brennan Johnson (Nottingham Forest)
ENGLAND
Most valuable player:
Phil Foden (£95.1m)
Overall squad value:
£1.03bn
Most capped player:
Raheem Sterling (81)
Leading goalscorer:
Harry Kane (51)
Total caps:
850
Total goals:
124
Oldest player:
Jordan Henderson (32)
Youngest player:
Jude Bellingham (19)
Average age:
26.5
SQUAD IN FULL
Goalkeepers: Jordan Pickford (Everton), Nick Pope (Newcastle United), Aaron Ramsdale (Arsenal)
Defenders: Trent Alexander-Arnold (Liverpool), Conor Coady (Everton, loan from Wolverhampton Wanderers), Eric Dier (Tottenham Hotspur), Harry Maguire (Manchester United), Luke Shaw (Manchester United), John Stones (Manchester City), Kieran Trippier (Newcastle United), Kyle Walker (Manchester City), Ben White (Arsenal)
Midfielders: Jude Bellingham (Borussia Dortmund), Conor Gallagher (Chelsea), Jordan Henderson (Liverpool), Mason Mount (Chelsea), Kalvin Phillips (Manchester City), Declan Rice (West Ham United)
Forwards: Phil Foden (Manchester City), Jack Grealish (Manchester City), Harry Kane (Tottenham Hotspur), James Maddison (Leicester City), Marcus Rashford (Manchester United), Bukayo Saka (Arsenal), Raheem Sterling (Chelsea), Callum Wilson (Newcastle United)
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