Jalel Kadri's men secured an impressive 0-0 draw against Denmark in their opener last time out, while Graham Arnold's men were on the receiving end of a 4-1 thrashing against current world champions France.
Defeat for either of Saturday's opponents would all but spell the end of their World Cup campaign, and we are expecting a thrilling encounter in this one.
Here, Sports Mole looks at how the squads of Tunisia and Australia stack up against each other ahead of the contest.
TUNISIA
Most valuable player:
Ellyes Skhiri (£11.3m)
Overall squad value:
£54.1m
Most capped player:
Youssef Msakni (89)
Leading goalscorer:
Wahbi Khazri (24)
Total caps:
1001
Total goals:
105
Oldest player:
Aymen Balbouli (38)
Youngest player:
Hannibal Mejbri (19)
Average age:
27.8
SQUAD IN FULL
Goalkeepers: Aymen Dahmen (Sfax), Bechir Ben Said (Monastir), Aymen Balbouli (Etoile du Sahel), Mouez Hassan (Club Africain)
Defenders: Mohamed Drager (Luzern), Wajdi Kechrida (Atromitos), Montassar Talbi (Lorient), Dylan Bronn (Salernitana), Nade Ghandri (Club Africain), Yassine Meriah (ES Tunis), Bilel Ifa (Al-Kuwait), Ali Maaloul (Al-Ahly), Ali Abdi (Caen)
Midfielders: Aissa Laidouni (Ferencvaros), Ellis Skhiri (FC Cologne), Ghaylen Chaaleli (ES Tunis), Mohamed Ali Ben Romdhane (ES Tunis), Hannibal Mejbri (Birmingham City), Ferjani Sassi (Al-Duhail), Anis Ben Slimane (Brondby)
Forwards: Naim Sliti (Ettifaq), Wahbi Khazri (Montpellier), Saifeddine Jaziri (Zamalek), Yassine Khenissi (Al-Kuwait), Youssef Msakni (Al-Arabi), Issam Jebali (Odense)
AUSTRALIA
Most valuable player:
Mathew Ryan (£4.3m)
Overall squad value:
£32.3m
Most capped player:
Mathew Ryan (76)
Leading goalscorer:
Mathew Leckie (13)
Total caps:
553
Total goals:
70
Oldest player:
Danny Vukovic (37)
Youngest player:
Garang Kuol (18)
Average age:
27.2
SQUAD IN FULL
Goalkeepers: Mathew Ryan (Copenhagen), Danny Vukovic (Central Coast Mariners), Andrew Redmayne (Sydney FC)
Defenders: Aziz Behich (Dundee United), Milos Degenek (Columbus Crew), Bailey Wright (Sunderland), Fran Karacic (Brescia), Harry Souttar (Stoke City), Nathaniel Atkinson (Hearts), Joel King (Odense), Kyle Rowles (Hearts), Thomas Deng (Albirex Niigata)
Midfielders: Aaron Mooy (Celtic), Jackson Irvine (St Pauli), Ajdin Hrustic (Hellas Verona), Riley McGree (Middlesbrough), Keanu Baccus (St Mirren), Cameron Devlin (Hearts)
Forwards: Mathew Leckie (Melbourne City), Awer Mabil (Cadiz), Jamie Maclaren (Melbourne City), Mitchell Duke (Fagiano Okayama), Martin Boyle (Hibernian), Craig Goodwin (Adelaide United), Jason Cummings (Central Coast Mariners), Garang Kuol (Central Coast Mariners)
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How Tunisia could line up against Australia
How Australia could line up against Tunisia