England superstar Kane has reportedly asked to leave the club this summer amid their struggles to win silverware again this season.
Chelsea's win over Leicester last night has also meant that Spurs have missed out on Champions League football.
Ryan Mason's side will want to secure Europa League football for next term and a win tonight is vital.
Villa, who lost 2-0 to Tottenham in March, are set for a mid-table finish.
Tottenham v Aston Villa: How to listen
This match will get under way at 6pm on Wednesday, May 19.
Russ Williams will be your host and commentary will come from Joe Shennan and Dean Ashton.
To tune in, click HERE for the live stream.
Tottenham v Aston Villa: Team news
Harry Kane will continue to lead Tottenham's line against Aston Villa amid fresh speculation surrounding his future.
Interim boss Ryan Mason has confirmed he will start against Villa despite the speculation surrounding his future.
Gareth Bale is a doubt due to fatigue while Serge Aurier and Ben Davies remain out.
Tyrone Mings' knee injury will be assessed ahead of kick-off.
Morgan Sanson will sit out the final two games of the season after suffering a reoccurrence of his knee problem.
Matty Cash is also sidelined until next term with Trezeguet unlikely to feature until the end of the year due to a serious knee injury.
Tottenham v Aston Villa: What has been said?
Interim Tottenham boss Ryan Mason says he cannot answer questions on Harry Kane's future because he will not be the manager next season.
"I'm not uncomfortable at all. I couldn't be more comfortable," he said. "I'm at the club I love. I've got people around me who are great. I feel at home here.
"So answering questions comes with the role, it's normal.
"I've been given the duty of taking the football club for the remaining seven games of the season, so there are certain questions I can't answer because I don't know because I'm not the manager for the future - so I can't answer the questions.
"I'm not in those conversations about next season, what the squad's going to look like, who we want to bring in because my focus in on the next two games.
"So in terms of being uncomfortable, no. I'm really comfortable.
"I've been given this opportunity and I feel I wouldn't have been handed this opportunity if the club didn't feel like I was ready to help the team and Tottenham Hotspur.
"When you represent a big club there is always going to be speculation. Football when you lose, it's horrible, when you win it's great.
"In terms of a crash-course, you could say that. But its something I feel comfortable dealing with."
Tottenham v Aston Villa: Match stats and facts
Tottenham are looking to complete their seventh Premier League double over Aston Villa, something they've only done as many times in the competition against Everton (7) and Manchester City (8).
Aston Villa have lost 12 of their last 13 meetings with Tottenham in all competitions, winning the other in April 2015 under Tim Sherwood.
Tottenham have won their final home league game in 10 of the last 12 seasons, with the exceptions being a 1-2 loss to Southampton in 2015-16 and a 2-2 draw with Everton in 2018-19.
Aston Villa have won their final away league game in just one of the last 18 seasons (D7 L10), beating Arsenal 2-1 at the Emirates in 2010-11.
Tottenham have won 11 of their last 12 Premier League home games played on Wednesdays (D1) since a 0-1 defeat against Leicester City in January 2016.
Aston Villa have lost all five of their Premier League games played on Wednesdays this season - the most games a team has played on a specific day of the week within a single season and lost them all is six (Tottenham on Sundays in 1996-97).
Tottenham striker Harry Kane has scored seven goals in his last six Premier League appearances against Aston Villa, finding the net in each of his last four against the Villans.
Aston Villa boss Dean Smith has lost each of his three Premier League matches against Spurs - he's only lost his first four league encounters against two sides in his managerial career (Man City and Blackburn).
This match will be Harry Kane's 400th appearance in club football in all competitions - 334 of his 399 so far have come for Spurs, with the others coming during loan spells at Leyton Orient (18), Millwall (27), Norwich City (5) and Leicester City (15). His 236 goals since his debut in January 2011 are bettered only by Sergio Agüero (258) in English football in that time.
Aston Villa midfielder John McGinn scored his sixth Premier League goal in their 3-2 defeat at Crystal Palace last time out - the Scotsman has scored the opening goal in four Premier League games and Villa have gone on to lose all four. Only former Bolton striker Ivan Klasnic has scored the opening goal in more Premier League games without winning than McGinn (5 - drawing all five).