Dragan Stojkovic's men currently sit bottom of the group and need to come away with all three points in this one to stand any chance of progressing to the last 16.
Murat Yakin's men, meanwhile, are in pole position to finish as runners-up behind Brazil, although anything less than a victory in their final outing would leave them looking anxiously over their shoulders.
Here Sports Mole looks at how the squads of Serbia and Switzerland weigh up against each other ahead of Friday's contest.
SERBA
Most valuable player:
Dusan Vlahovic (£69.2m)
Overall squad value:
£310.9m
Most capped player:
Dusan Tadic (93)
Leading goalscorer:
Aleksandar Mitrovic (51)
Total caps:
806
Total goals:
120
Oldest player:
Dusan Tadic (34)
Youngest player:
Strahinja Pavlovic (21)
Average age:
26.9
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Goalkeepers: Marko Dmitrovic (Sevilla), Predrag Rajkovic (Mallorca), Vanja Milinkovic-Savic (Torino)
Defenders: Stefan Mitrovic (Getafe), Nikola Milenkovic (Fiorentina), Strahinja Pavlovic (RB Salzburg), Milos Veljkovic (Werder Bremen), Filip Mladenovic (Legia Warsaw), Strahinja Erakovic (Crvena Zvezda), Srdjan Babic (Almeria)
Midfielders: Nemanja Gudelj (Sevilla), Sergej Milinkovic-Savic (Lazio), Sasa Lukic (Torino), Marko Grujic (Porto), Filip Kostic (Juventus), Uros Racic (Braga), Nemanja Maksimovic (Getafe), Ivan Ilic (Hellas Verona), Andrija Zivkovic (PAOK), Darko Lazovic (Hellas Verona)
Forwards: Dusan Tadic (Ajax), Aleksandar Mitrovic (Fulham), Dusan Vlahovic (Juventus), Filip Djuricic (Sampdoria), Luka Jovic (Fiorentina), Nemanja Radonjic (Torino)
SWITZERLAND
Most valuable player:
Manuel Akanji (£25.8m)
Overall squad value:
£242.3m
Most capped player:
Xherdan Shaqiri (110)
Leading goalscorer:
Xherdan Shaqiri (26)
Total caps:
1042
Total goals:
120
Oldest player:
Yann Sommer (33)
Youngest player:
Ardon Jashari (20)
Average age:
27
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Goalkeepers: Yann Sommer (Borussia Monchengladbach), Gregor Kobel (Borussia Dortmund), Jonas Omlin (Montpellier), Philipp Kohn (Red Bull Salzburg)
Defenders: Manuel Akanji (Manchester City), Eray Comert (Valencia), Nico Elvedi (Borussia Monchengladbach), Ricardo Rodriguez (Torino), Fabian Schar (Newcastle United), Silvan Widmer (Mainz 05)
Midfielders: Michel Aebischer (Bologna), Edimilson Fernandes (Mainz 05), Fabian Frei (Basel), Remo Freuler (Nottingham Forest), Ardon Jashari (Luzern), Fabian Rieder (Young Boys), Xherdan Shaqiri (Chicago Fire), Djibril Sow (Eintracht Frankfurt), Renato Steffen (Lugano), Granit Xhaka (Arsenal), Denis Zakaria (Chelsea)
Forwards: Breel Embolo (Monaco), Christian Fassnacht (Young Boys), Noah Okafor (Red Bull Salzburg), Haris Seferovic (Galatasaray), Ruben Vargas (FC Augsburg)
Read more on Serbia vs Switzerland
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